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PDBsum entry 2sbt
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Hydrolase (serine proteinase) PDB id
2sbt

 

 

 

 

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Contents
Protein chain
275 a.a. *
Ligands
ACN
Waters ×10
* Residue conservation analysis
PDB id:
2sbt
Name: Hydrolase (serine proteinase)
Title: A comparison of the three-dimensional structures of subtilisin bpn and subtilisin novo
Structure: Subtilisin novo. Chain: a. Engineered: yes
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Resolution:
2.80Å     R-factor:   not given    
Authors: J.Drenth,W.G.J.Hol,J.N.Jansonius,R.Koekoek
Key ref: J.Drenth et al. (1972). A comparison of the three-dimensional structures of subtilisin BPN' and subtilisin novo. Cold Spring Harb Symp Quant Biol, 36, 107-116. PubMed id: 4508127
Date:
07-Sep-76     Release date:   06-Oct-76    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00782  (SUBT_BACAM) -  Subtilisin BPN' from Bacillus amyloliquefaciens
Seq:
Struc: 		382 a.a.
275 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 10 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.4.21.62  - subtilisin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

 

 
Cold Spring Harb Symp Quant Biol 36:107-116 (1972)
PubMed id: 4508127  
 
 
A comparison of the three-dimensional structures of subtilisin BPN' and subtilisin novo.
J.Drenth, W.G.Hol, J.N.Jansonius, R.Koekoek.
 
  ABSTRACT  
 

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
12649438 R.M.Day, C.J.Thalhauser, J.L.Sudmeier, M.P.Vincent, E.V.Torchilin, D.G.Sanford, C.W.Bachovchin, and W.W.Bachovchin (2003).
Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN' by NMR.
  Protein Sci, 12, 794-810.  
8534804 J.Antosiewicz (1995).
Computation of the dipole moments of proteins.
  Biophys J, 69, 1344-1354.  
1384034 C.Eigenbrot, M.Randal, and A.A.Kossiakoff (1992).
Structural effects induced by mutagenesis affected by crystal packing factors: the structure of a 30-51 disulfide mutant of basic pancreatic trypsin inhibitor.
  Proteins, 14, 75-87.
PDB code: 1aal
275827 J.S.Richardson, E.D.Getzoff, and D.C.Richardson (1978).
The beta bulge: a common small unit of nonrepetitive protein structure.
  Proc Natl Acad Sci U S A, 75, 2574-2578.  
1064867 B.Honig, A.Ray, and C.Levinthal (1976).
Conformational flexibility and protein folding: rigid structural fragments connected by flexible joints in subtilisin BPN.
  Proc Natl Acad Sci U S A, 73, 1974-1978.  
164664 S.T.Thompson, K.H.Cass, and E.Stellwagen (1975).
Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.
  Proc Natl Acad Sci U S A, 72, 669-672.  
4351801 D.B.Wetlaufer (1973).
Nucleation, rapid folding, and globular intrachain regions in proteins.
  Proc Natl Acad Sci U S A, 70, 697-701.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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