PDBsum entry 2ftp

Lyase

PDB id: 2ftp

Contents

Protein chain

300 a.a. *

Ligands

GOL ×2

Metals

_NA

Waters ×222

* Residue conservation analysis

PDB id:

2ftp

Name:

Lyase

Title:

Crystal structure of hydroxymethylglutaryl-coa lyase from pseudomonas aeruginosa

Structure:

Hydroxymethylglutaryl-coa lyase. Chain: a. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PDB file)

Resolution:

2.40Å R-factor: 0.188 R-free: 0.213

Authors:

T.Xiao,E.Evdokimova,Y.Liu,M.Kudritska,A.Savchenko,E.F.Pai,A.Edwards, Midwest Center For Structural Genomics (Mcsg)

Key ref:

T.Xiao et al. Crystal structure of hydroxymethylglutaryl-Coa lyase from pseudomonas aeruginosa. To be published, .

Date:

24-Jan-06 Release date: 14-Mar-06

Protein chain

Q9I2A0  (LIUE_PSEAE) -  3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 300 a.a.

Struc: 300 a.a.

Enzyme reactions

• Enzyme class 1: E.C.4.1.3.26 - 3-hydroxy-3-isohexenylglutaryl-CoA lyase.
• Reaction: 3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA = 7-methyl-3-oxooct-6- enoyl-CoA + acetate

3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA = 7-methyl-3-oxooct-6- enoyl-CoA + acetate (Bound ligand (Het Group name = GOL) matches with 42.86% similarity)

• Enzyme class 2: E.C.4.1.3.4 - hydroxymethylglutaryl-CoA lyase.

Pathway:

• Reaction: (3S)-3-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA

(3S)-3-hydroxy-3-methylglutaryl-CoA = acetoacetate (Bound ligand (Het Group name = GOL) matches with 62.50% similarity) + acetyl-CoA

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site