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UniProt functional annotation for Q9I2A0 | ||
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| UniProt code: Q9I2A0. |
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| Organism: | |
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
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| Function: | |
Involved in the L-leucine, isovalerate and acyclic monoterpene catabolism. Catalyzes the cleavage of 3-hydroxy-3- methylglutaryl-CoA (HMG-CoA) to yield acetyl-CoA and acetoacetate. It can also catalyze the cleavage of 3-hydroxy-3-isohexenylglutaryl-CoA (HIHG_CoA) to yield 7-methyl-3-oxooct-6-enoyl-CoA and acetate. {ECO:0000269|PubMed:19459965, ECO:0000269|PubMed:19597963}. |
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| Catalytic activity: | |
Reaction=3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA = 7-methyl-3- oxooct-6-enoyl-CoA + acetate; Xref=Rhea:RHEA:23084, ChEBI:CHEBI:30089, ChEBI:CHEBI:57341, ChEBI:CHEBI:71410; EC=4.1.3.26; Evidence={ECO:0000269|PubMed:19459965, ECO:0000269|PubMed:19597963}; |
| Catalytic activity: | |
Reaction=(3S)-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA; Xref=Rhea:RHEA:24404, ChEBI:CHEBI:13705, ChEBI:CHEBI:43074, ChEBI:CHEBI:57288; EC=4.1.3.4; Evidence={ECO:0000269|PubMed:19597963}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:19459965}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:19459965}; Note=Divalent cations such as magnesium or manganese. {ECO:0000269|PubMed:19459965}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=100 uM for HMG-CoA (at pH 6.7 and 37 degrees Celsius) {ECO:0000269|PubMed:19459965}; Vmax=21.7 umol/min/mg enzyme (at pH 6.7 and 37 degrees Celsius) {ECO:0000269|PubMed:19459965}; pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:19459965}; Temperature dependence: Optimum temperature is 37 degrees Celsius. {ECO:0000269|PubMed:19459965}; |
| Pathway: | |
Metabolic intermediate metabolism; (S)-3-hydroxy-3- methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3- methylglutaryl-CoA: step 1/1. {ECO:0000305}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:19459965}. |
| Disruption phenotype: | |
Cells lacking this gene are unable to utilize L- leucine, isovalerate or acyclic terpenes as carbon source. {ECO:0000269|PubMed:19459965}. |
| Similarity: | |
Belongs to the HMG-CoA lyase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.