PDBsum entry 2fio

Transcription/DNA

PDB id: 2fio

Contents

Protein chains

123 a.a.

113 a.a.

DNA/RNA

Waters ×86

PDB id:

2fio

Name:

Transcription/DNA

Title:

Phage phi29 transcription regulator p4-DNA complex

Structure:

DNA (41-mer). Chain: c. Engineered: yes. DNA (41-mer). Chain: d. Engineered: yes. Late genes activator. Chain: a, b. Synonym: early protein gp4.

Source:

Synthetic: yes. Bacillus phage phi29. Organism_taxid: 10756. Gene: 4. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Tetramer (from PQS)

Resolution:

2.70Å R-factor: 0.229 R-free: 0.279

Authors:

D.Badia,A.Camacho,L.Perez-Lago,C.Escandon,M.Salas,M.Coll

Key ref:

D.Badia et al. (2006). The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA. Mol Cell, 22, 73-81. PubMed id: 16600871 DOI: 10.1016/j.molcel.2006.02.019

Date:

30-Dec-05 Release date: 26-Sep-06

Protein chain

P03682  (TF4_BPPH2) -  Late genes activator p4 from Bacillus phage phi29

Seq: 125 a.a.

Struc: 123 a.a.

Protein chain

P03682  (TF4_BPPH2) -  Late genes activator p4 from Bacillus phage phi29

Seq: 125 a.a.

Struc: 113 a.a.

DNA/RNA chains

A-A-A-A-A-C-G-T-C-A-A-C-A-T-T-T-T-A-T-A-A-A-A-A-A-G-T-C-T-T-G-C-A-A-A-A-A-G-T- 41 bases

T-A-A-C-T-T-T-T-T-G-C-A-A-G-A-C-T-T-T-T-T-T-A-T-A-A-A-A-T-G-T-T-G-A-C-G-T-T-T- 41 bases

Enzyme reactions

• Enzyme class: Chains A, B: E.C.?

DOI no: 10.1016/j.molcel.2006.02.019

Mol Cell 22:73-81 (2006)

PubMed id: 16600871

The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA.

D.Badia, A.Camacho, L.Pérez-Lago, C.Escandón, M.Salas, M.Coll.

ABSTRACT

Protein p4 affects the transcriptional switch that divides bacteriophage phi29 infection in early and late phases. The synthesis of DNA replication proteins and p4 takes place in the early phase, while structural, morphogenesis, and lysis proteins are synthesized in the late phase. Transcriptional switch by p4 is achieved by activating the late promoter A3 and repressing the early promoters A2b and A2c. The crystal structure of p4 alone and in complex with a 41 bp DNA, including the A3 promoter binding site, helps us to understand how the phage cycle is controlled. Protein p4 has a unique alpha/beta fold that includes a DNA recognition motif consisting of two N-terminal beta turn substructures, or N-hooks, located at the tips of an elongated protein homodimer. The two N-hooks enter the major groove of the double helix, establishing base-specific contacts. A high DNA curvature allows p4 N-hooks to reach two major groove areas three helical turns apart, like a bow and its string.

Selected figure(s)

Figure 2.
Figure 2. Structure of the p4 Monomer and the N-Hook

Figure 3.
Figure 3. Structure of the p4 Dimer-DNA Complex

The above figures are reprinted by permission from Cell Press: Mol Cell (2006, 22, 73-81) copyright 2006.

Figures were selected by an automated process.