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PDBsum entry 2fio
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Transcription/DNA
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PDB id
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2fio
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References listed in PDB file
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Key reference
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Title
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The structure of phage phi29 transcription regulator p4-Dna complex reveals an n-Hook motif for DNA.
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Authors
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D.Badia,
A.Camacho,
L.Pérez-Lago,
C.Escandón,
M.Salas,
M.Coll.
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Ref.
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Mol Cell, 2006,
22,
73-81.
[DOI no: ]
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PubMed id
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Abstract
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Protein p4 affects the transcriptional switch that divides bacteriophage phi29
infection in early and late phases. The synthesis of DNA replication proteins
and p4 takes place in the early phase, while structural, morphogenesis, and
lysis proteins are synthesized in the late phase. Transcriptional switch by p4
is achieved by activating the late promoter A3 and repressing the early
promoters A2b and A2c. The crystal structure of p4 alone and in complex with a
41 bp DNA, including the A3 promoter binding site, helps us to understand how
the phage cycle is controlled. Protein p4 has a unique alpha/beta fold that
includes a DNA recognition motif consisting of two N-terminal beta turn
substructures, or N-hooks, located at the tips of an elongated protein
homodimer. The two N-hooks enter the major groove of the double helix,
establishing base-specific contacts. A high DNA curvature allows p4 N-hooks to
reach two major groove areas three helical turns apart, like a bow and its
string.
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Figure 2.
Figure 2. Structure of the p4 Monomer and the N-Hook
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Figure 3.
Figure 3. Structure of the p4 Dimer-DNA Complex
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The above figures are
reprinted
by permission from Cell Press:
Mol Cell
(2006,
22,
73-81)
copyright 2006.
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