PDBsum entry 1xnh

Ligase

PDB id: 1xnh

Contents

Protein chain

251 a.a. *

Waters ×37

* Residue conservation analysis

PDB id:

1xnh

Name:

Ligase

Title:

Crystal structure of nh3-dependent NAD+ synthetase from helicobacter pylori

Structure:

Nh(3)-dependent NAD(+) synthetase. Chain: a. Engineered: yes

Source:

Helicobacter pylori. Organism_taxid: 210. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PDB file)

Resolution:

2.30Å R-factor: 0.255 R-free: 0.295

Authors:

G.B.Kang,Y.S.Kim,Y.J.Im,S.H.Rho,J.H.Lee,S.H.Eom

Key ref:

G.B.Kang et al. (2005). Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori. Proteins, 58, 985-988. PubMed id: 15645437 DOI: 10.1002/prot.20377

Date:

05-Oct-04 Release date: 05-Apr-05

Protein chain

O25096  (NADE_HELPY) -  NH(3)-dependent NAD(+) synthetase from Helicobacter pylori (strain ATCC 700392 / 26695)

Seq: 260 a.a.

Struc: 251 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.1.5 - NAD(+) synthase.
• Reaction: deamido-NAD⁺ + NH4⁺ + ATP = AMP + diphosphate + NAD⁺ + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1002/prot.20377

Proteins 58:985-988 (2005)

PubMed id: 15645437

Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori.

G.B.Kang, Y.S.Kim, Y.J.Im, S.H.Rho, J.H.Lee, S.H.Eom.

ABSTRACT

No abstract given.

Selected figure(s)

Figure 1.
Figure 1. Structure of H. pylori NAD^+ synthetase. (A) Stereo view of a ribbon diagram showing the dimeric structure of NAD^+ synthetase. The -helices are labeled A- L, and the -strands are labeled A- D. The two subunits are colored green and blue. The bound NaAD and ATP molecules are shown as the ball-and-stick models. The red line indicates a flexible loop region (residued 179-191). (B) Stereo view of the ATP binding site. Residues of the P-loop are colored cyan. (C) Stereo view of the NaAD binding site.

Figure 2.
Figure 2. The multiple sequence alignment of H. pylori, B. subtilis, and human NAD^+ synthetases. Sequences were aligned using ClustalX. Highly conserved residues are shaded in black and gray. The secondary structure elements of NAD^+ synthetase are indicated by arrows ( -strands), bars ( -helices), and lines (connecting loop). Residues constituting ATP and NaAD binding sites are indicated by open and filled circles, respectively.

The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2005, 58, 985-988) copyright 2005.