PDBsum entry 1vbi

Oxidoreductase

PDB id: 1vbi

Contents

Protein chain

340 a.a. *

Ligands

SO4 ×3

NAD

EDO ×4

Waters ×342

* Residue conservation analysis

PDB id:

1vbi

Name:

Oxidoreductase

Title:

Crystal structure of type 2 malate/lactate dehydrogenase from thermus thermophilus hb8

Structure:

Type 2 malate/lactate dehydrogenase. Chain: a. Engineered: yes

Source:

Thermus thermophilus. Organism_taxid: 274. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PDB file)

Resolution:

1.80Å R-factor: 0.187 R-free: 0.210

Authors:

H.Mizutani,N.Kunishima,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

H.Mizutani and n.kunishima Crystal structure of type 2 malate/lactate dehydrogenase from thermus thermophilus hb8. To be published, .

Date:

27-Feb-04 Release date: 19-Apr-05

Protein chain

Q53W84  (Q53W84_THET8) -  Malate/L-lactate dehydrogenase family protein from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 344 a.a.

Struc: 340 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.27 - L-lactate dehydrogenase.
• Reaction: (S)-lactate + NAD⁺ = pyruvate + NADH + H⁺

(S)-lactate (Bound ligand (Het Group name = EDO) matches with 66.67% similarity) + NAD(+) (Bound ligand (Het Group name = NAD) corresponds exactly) = pyruvate + NADH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site