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PDBsum entry 1omc
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Presynaptic neurotoxin PDB id
1omc

 

 

 

 

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Contents
Protein chain
28 a.a.
PDB id:
1omc
Name: Presynaptic neurotoxin
Title: Solution structure of omega-conotoxin gvia using 2-d nmr spectroscopy and relaxation matrix analysis
Structure: Omega-conotoxin gvia. Chain: a. Engineered: yes
Source: Conus geographus. Geography cone. Organism_taxid: 6491
NMR struc: 21 models
Authors: J.H.Davis,E.K.Bradley,G.P.Miljanich,L.Nadasdi,J.Ramachandran, V.J.Basus
Key ref:
J.H.Davis et al. (1993). Solution structure of omega-conotoxin GVIA using 2-D NMR spectroscopy and relaxation matrix analysis. Biochemistry, 32, 7396-7405. PubMed id: 8338837 DOI: 10.1021/bi00080a009
Date:
28-Apr-93     Release date:   31-Jan-94    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P01522  (O16A_CONGE) -  Omega-conotoxin GVIA from Conus geographus
Seq:
Struc: 		73 a.a.
28 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 

 
DOI no: 10.1021/bi00080a009 Biochemistry 32:7396-7405 (1993)
PubMed id: 8338837  
 
 
Solution structure of omega-conotoxin GVIA using 2-D NMR spectroscopy and relaxation matrix analysis.
J.H.Davis, E.K.Bradley, G.P.Miljanich, L.Nadasdi, J.Ramachandran, V.J.Basus.
 
  ABSTRACT  
 
We report here the solution structure of omega-conotoxin GVIA, a peptide antagonist of the N-type neuronal voltage-sensitive calcium channel. The structure was determined using two-dimensional NMR in combination with distance geometry and restrained molecular dynamics. The full relaxation matrix analysis program MARDIGRAS was used to generate maximum and minimum distance restraints from the crosspeak intensities in NOESY spectra. The 187 restraints obtained were used in conjunction with 23 angle restraints from vicinal coupling constants as input for the structure calculations. The backbones of the best 21 structures match with an average pairwise RMSD of 0.58 A. The structures contain a short segment of triple-stranded beta-sheet involving residues 6-8, 18-21, and 24-27, making this the smallest published peptide structure to contain a triple-stranded beta-sheet. Conotoxins have been shown to be effective neuroprotective agents in animal models of brain ischemia. Our results should aid in the design of novel nonpeptide compounds with potential therapeutic utility.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20175537 K.H.Gowd, V.Yarotskyy, K.S.Elmslie, J.J.Skalicky, B.M.Olivera, and G.Bulaj (2010).
Site-specific effects of diselenide bridges on the oxidative folding of a cystine knot peptide, omega-selenoconotoxin GVIA.
  Biochemistry, 49, 2741-2752.  
16756550 L.Shi, B.Li, and S.M.Paskewitz (2006).
Cloning and characterization of a putative inhibitor of melanization from Anopheles gambiae.
  Insect Mol Biol, 15, 313-320.  
14696181 C.Bernard, G.Corzo, S.Adachi-Akahane, G.Foures, K.Kanemaru, Y.Furukawa, T.Nakajima, and H.Darbon (2004).
Solution structure of ADO1, a toxin extracted from the saliva of the assassin bug, Agriosphodrus dohrni.
  Proteins, 54, 195-205.
PDB code: 1lmr
14681383 J.C.Gelly, J.Gracy, Q.Kaas, D.Le-Nguyen, A.Heitz, and L.Chiche (2004).
The KNOTTIN website and database: a new information system dedicated to the knottin scaffold.
  Nucleic Acids Res, 32, D156-D159.  
14976206 L.Volpon, H.Lamthanh, J.Barbier, N.Gilles, J.Molgó, A.Ménez, and J.M.Lancelin (2004).
NMR solution structures of delta-conotoxin EVIA from Conus ermineus that selectively acts on vertebrate neuronal Na+ channels.
  J Biol Chem, 279, 21356-21366.
PDB codes: 1g1p 1g1z
14556622 L.Jouvensal, L.Quillien, E.Ferrasson, Y.Rahbé, J.Guéguen, and F.Vovelle (2003).
PA1b, an insecticidal protein extracted from pea seeds (Pisum sativum): 1H-2-D NMR study and molecular modeling.
  Biochemistry, 42, 11915-11923.
PDB code: 1p8b
11876659 M.Price-Carter, G.Bulaj, and D.P.Goldenberg (2002).
Initial disulfide formation steps in the folding of an omega-conotoxin.
  Biochemistry, 41, 3507-3519.  
11344322 D.P.Goldenberg, R.E.Koehn, D.E.Gilbert, and G.Wagner (2001).
Solution structure and backbone dynamics of an omega-conotoxin precursor.
  Protein Sci, 10, 538-550.
PDB code: 1feo
11478874 S.Ludvigsen, L.Thim, A.M.Blom, and B.S.Wulff (2001).
Solution structure of the satiety factor, CART, reveals new functionality of a well-known fold.
  Biochemistry, 40, 9082-9088.
PDB code: 1hy9
10903497 J.M.Hill, P.F.Alewood, and D.J.Craik (2000).
Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure.
  Eur J Biochem, 267, 4649-4657.
PDB code: 1eyo
10822250 K.J.Nielsen, T.Schroeder, and R.Lewis (2000).
Structure-activity relationships of omega-conotoxins at N-type voltage-sensitive calcium channels.
  J Mol Recognit, 13, 55-70.  
11101291 K.Kobayashi, T.Sasaki, K.Sato, and T.Kohno (2000).
Three-dimensional solution structure of omega-conotoxin TxVII, an L-type calcium channel blocker.
  Biochemistry, 39, 14761-14767.
PDB code: 1f3k
10026302 A.C.Daquinag, T.Sato, H.Koda, T.Takao, M.Fukuda, Y.Shimonishi, and T.Tsukamoto (1999).
A novel endogenous inhibitor of phenoloxidase from Musca domestica has a cystine motif commonly found in snail and spider toxins.
  Biochemistry, 38, 2179-2188.  
10081964 B.Gilquin, A.Lecoq, F.Desné, M.Guenneugues, S.Zinn-Justin, and A.Ménez (1999).
Conformational and functional variability supported by the BPTI fold: solution structure of the Ca2+ channel blocker calcicludine.
  Proteins, 34, 520-532.
PDB code: 1bf0
10400675 S.Lu, P.Deng, X.Liu, J.Luo, R.Han, X.Gu, S.Liang, X.Wang, F.Li, V.Lozanov, A.Patthy, and S.Pongor (1999).
Solution structure of the major alpha-amylase inhibitor of the crop plant amaranth.
  J Biol Chem, 274, 20473-20478.
PDB code: 1qfd
9657699 M.Price-Carter, M.S.Hull, and D.P.Goldenberg (1998).
Roles of individual disulfide bonds in the stability and folding of an omega-conotoxin.
  Biochemistry, 37, 9851-9861.  
9548922 P.Savarin, M.Guenneugues, B.Gilquin, H.Lamthanh, S.Gasparini, S.Zinn-Justin, and A.Ménez (1998).
Three-dimensional structure of kappa-conotoxin PVIIA, a novel potassium channel-blocking toxin from cone snails.
  Biochemistry, 37, 5407-5416.
PDB code: 1kcp
9477946 S.S.Mitchell, K.J.Shon, M.P.Foster, D.R.Davis, B.M.Olivera, and C.M.Ireland (1998).
Three-dimensional solution structure of conotoxin psi-PIIIE, an acetylcholine gated ion channel antagonist.
  Biochemistry, 37, 1215-1220.
PDB code: 1as5
9115446 J.M.Hill, P.F.Alewood, and D.J.Craik (1997).
Solution structure of the sodium channel antagonist conotoxin GS: a new molecular caliper for probing sodium channel geometry.
  Structure, 5, 571-583.
PDB code: 1ag7
9048550 K.H.Han, K.J.Hwang, S.M.Kim, S.K.Kim, W.R.Gray, B.M.Olivera, J.Rivier, and K.J.Shon (1997).
NMR structure determination of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA.
  Biochemistry, 36, 1669-1677.
PDB code: 1p1p
9144657 R.H.Scott, V.J.Gorton, L.Harding, D.Patel, S.Pacey, C.Kellenberger, H.Hietter, and I.Bermudez (1997).
Inhibition of neuronal high voltage-activated calcium channels by insect peptides: a comparison with the actions of omega-conotoxin GVIA.
  Neuropharmacology, 36, 195-208.  
8608119 D.O.Omecinsky, K.E.Holub, M.E.Adams, and M.D.Reily (1996).
Three-dimensional structure analysis of mu-agatoxins: further evidence for common motifs among neurotoxins with diverse ion channel specificities.
  Biochemistry, 35, 2836-2844.
PDB codes: 1eit 1eiu 1eiv
8952508 M.Price-Carter, W.R.Gray, and D.P.Goldenberg (1996).
Folding of omega-conotoxins. 1. Efficient disulfide-coupled folding of mature sequences in vitro.
  Biochemistry, 35, 15537-15546.  
8952509 M.Price-Carter, W.R.Gray, and D.P.Goldenberg (1996).
Folding of omega-conotoxins. 2. Influence of precursor sequences and protein disulfide isomerase.
  Biochemistry, 35, 15547-15557.  
8987986 M.Prorok, S.E.Warder, T.Blandl, and F.J.Castellino (1996).
Calcium binding properties of synthetic gamma-carboxyglutamic acid-containing marine cone snail "sleeper" peptides, conantokin-G and conantokin-T.
  Biochemistry, 35, 16528-16534.  
8973180 T.Kohno, K.Kobayashi, T.Maeda, K.Sato, and A.Takashima (1996).
Three-dimensional structures of the amyloid beta peptide (25-35) in membrane-mimicking environment.
  Biochemistry, 35, 16094-16104.
PDB code: 1qcm
7546566 B.M.Olivera, D.R.Hillyard, M.Marsh, and D.Yoshikami (1995).
Combinatorial peptide libraries in drug design: lessons from venomous cone snails.
  Trends Biotechnol, 13, 422-426.  
9223017 J.P.Flinn, R.Murphy, J.H.Boublik, M.J.Lew, C.E.Wright, and J.A.Angus (1995).
Synthesis and biological characterization of a series of analogues of omega-conotoxin GVIA.
  J Pept Sci, 1, 379-384.  
  8528070 M.Sukumar, J.Rizo, M.Wall, L.A.Dreyfus, Y.M.Kupersztoch, and L.M.Gierasch (1995).
The structure of Escherichia coli heat-stable enterotoxin b by nuclear magnetic resonance and circular dichroism.
  Protein Sci, 4, 1718-1729.
PDB code: 1ehs
7773771 L.Narasimhan, J.Singh, C.Humblet, K.Guruprasad, and T.Blundell (1994).
Snail and spider toxins share a similar tertiary structure and 'cystine motif'.
  Nat Struct Biol, 1, 850-852.  
  7849598 P.K.Pallaghy, K.J.Nielsen, D.J.Craik, and R.S.Norton (1994).
A common structural motif incorporating a cystine knot and a triple-stranded beta-sheet in toxic and inhibitory polypeptides.
  Protein Sci, 3, 1833-1839.  
  8251934 J.J.Skalicky, W.J.Metzler, D.J.Ciesla, A.Galdes, and A.Pardi (1993).
Solution structure of the calcium channel antagonist omega-conotoxin GVIA.
  Protein Sci, 2, 1591-1603.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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