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PDBsum entry 1omc
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Presynaptic neurotoxin
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PDB id
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1omc
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References listed in PDB file
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Key reference
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Title
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Solution structure of omega-Conotoxin gvia using 2-D nmr spectroscopy and relaxation matrix analysis.
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Authors
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J.H.Davis,
E.K.Bradley,
G.P.Miljanich,
L.Nadasdi,
J.Ramachandran,
V.J.Basus.
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Ref.
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Biochemistry, 1993,
32,
7396-7405.
[DOI no: ]
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PubMed id
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Abstract
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We report here the solution structure of omega-conotoxin GVIA, a peptide
antagonist of the N-type neuronal voltage-sensitive calcium channel. The
structure was determined using two-dimensional NMR in combination with distance
geometry and restrained molecular dynamics. The full relaxation matrix analysis
program MARDIGRAS was used to generate maximum and minimum distance restraints
from the crosspeak intensities in NOESY spectra. The 187 restraints obtained
were used in conjunction with 23 angle restraints from vicinal coupling
constants as input for the structure calculations. The backbones of the best 21
structures match with an average pairwise RMSD of 0.58 A. The structures contain
a short segment of triple-stranded beta-sheet involving residues 6-8, 18-21, and
24-27, making this the smallest published peptide structure to contain a
triple-stranded beta-sheet. Conotoxins have been shown to be effective
neuroprotective agents in animal models of brain ischemia. Our results should
aid in the design of novel nonpeptide compounds with potential therapeutic
utility.
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Secondary reference #1
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Title
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Neuronal calcium channel inhibitors. Synthesis of omega-Conotoxin gvia and effects on 45ca uptake by synaptosomes.
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Authors
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J.Rivier,
R.Galyean,
W.R.Gray,
A.Azimi-Zonooz,
J.M.Mcintosh,
L.J.Cruz,
B.M.Olivera.
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Ref.
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J Biol Chem, 1987,
262,
1194-1198.
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PubMed id
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Secondary reference #2
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Title
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Purification and sequence of a presynaptic peptide toxin from conus geographus venom.
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Authors
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B.M.Olivera,
J.M.Mcintosh,
L.J.Cruz,
F.A.Luque,
W.R.Gray.
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Ref.
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Biochemistry, 1984,
23,
5087-5090.
[DOI no: ]
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PubMed id
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