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PDBsum entry 1koa
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Kinase PDB id
1koa

 

 

 

 

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Contents
Protein chain
447 a.a. *
* Residue conservation analysis
PDB id:
1koa
Name: Kinase
Title: Twitchin kinase fragment (c.Elegans), autoregulated protein kinase and immunoglobulin domains
Structure: Twitchin. Chain: a. Fragment: kinase fragment. Engineered: yes
Source: Caenorhabditis elegans. Organism_taxid: 6239. Expressed in: escherichia coli. Expression_system_taxid: 562
Biol. unit: Dimer (from PQS)
Resolution:
3.30Å     R-factor:   0.251     R-free:   0.351
Authors: B.Kobe,J.Heierhorst,S.C.Feil,M.W.Parker,G.M.Benian,K.R.Weiss,B.E.Kemp
Key ref: B.Kobe et al. (1996). Giant protein kinases: domain interactions and structural basis of autoregulation. Embo J, 15, 6810-6821. PubMed id: 9003756
Date:
28-Jun-96     Release date:   12-Mar-97    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q23551  (UNC22_CAEEL) -  Twitchin from Caenorhabditis elegans
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Struc: 		7158 a.a.
447 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.2.7.11.1  - non-specific serine/threonine protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H+
L-seryl-[protein]
 + ATP
 = O-phospho-L-seryl-[protein]
 + ADP
 + H(+)
L-threonyl-[protein]
 + ATP
 = O-phospho-L-threonyl-[protein]
 + ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Embo J 15:6810-6821 (1996)
PubMed id: 9003756  
 
 
Giant protein kinases: domain interactions and structural basis of autoregulation.
B.Kobe, J.Heierhorst, S.C.Feil, M.W.Parker, G.M.Benian, K.R.Weiss, B.E.Kemp.
 
  ABSTRACT  
 
The myosin-associated giant protein kinases twitchin and titin are composed predominantly of fibronectin- and immunoglobulin-like modules. We report the crystal structures of two autoinhibited twitchin kinase fragments, one from Aplysia and a larger fragment from Caenorhabditis elegans containing an additional C-terminal immunoglobulin-like domain. The structure of the longer fragment shows that the immunoglobulin domain contacts the protein kinase domain on the opposite side from the catalytic cleft, laterally exposing potential myosin binding residues. Together, the structures reveal the cooperative interactions between the autoregulatory region and the residues from the catalytic domain involved in protein substrate binding, ATP binding, catalysis and the activation loop, and explain the differences between the observed autoinhibitory mechanism and the one found in the structure of calmodulin-dependent kinase I.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21286791 T.M.Butler, and M.J.Siegman (2011).
A force-activated kinase in a catch smooth muscle.
  J Muscle Res Cell Motil, 31, 349-358.  
20196616 Y.Mabuchi, K.Mabuchi, W.F.Stafford, and Z.Grabarek (2010).
Modular structure of smooth muscle Myosin light chain kinase: hydrodynamic modeling and functional implications.
  Biochemistry, 49, 2903-2917.  
18390597 D.N.Greene, T.Garcia, R.B.Sutton, K.M.Gernert, G.M.Benian, and A.F.Oberhauser (2008).
Single-molecule force spectroscopy reveals a stepwise unfolding of Caenorhabditis elegans giant protein kinase domains.
  Biophys J, 95, 1360-1370.  
16917500 R.Jauch, M.K.Cho, S.Jäkel, C.Netter, K.Schreiter, B.Aicher, M.Zweckstetter, H.Jäckle, and M.C.Wahl (2006).
Mitogen-activated protein kinases interacting kinases are autoinhibited by a reprogrammed activation segment.
  EMBO J, 25, 4020-4032.
PDB codes: 2hw6 2hw7
15752366 A.G.Cook, L.N.Johnson, and J.M.McDonnell (2005).
Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide.
  FEBS J, 272, 1511-1522.  
15531631 F.Gräter, J.Shen, H.Jiang, M.Gautel, and H.Grubmüller (2005).
Mechanically induced titin kinase activation studied by force-probe molecular dynamics simulations.
  Biophys J, 88, 790-804.  
16341830 M.Marino, D.I.Svergun, L.Kreplak, P.V.Konarev, B.Maco, D.Labeit, and O.Mayans (2005).
Poly-Ig tandems from I-band titin share extended domain arrangements irrespective of the distinct features of their modular constituents.
  J Muscle Res Cell Motil, 26, 355-365.  
15771780 S.Cheek, K.Ginalski, H.Zhang, and N.V.Grishin (2005).
A comprehensive update of the sequence and structure classification of kinases.
  BMC Struct Biol, 5, 6.  
16453163 T.M.Ferrara, D.B.Flaherty, and G.M.Benian (2005).
Titin/connectin-related proteins in C. elegans: a review and new findings.
  J Muscle Res Cell Motil, 26, 435-447.  
15039579 A.Yamakawa, H.Ogata, K.Morita, N.Shibata, N.Andou, H.Sanuki, K.Yamada, T.Hioki, T.Ishii, and Y.Higuchi (2004).
Crystallization and preliminary X-ray analysis of two inhibitor complexes of the catalytic domain of death-associated protein kinase.
  Acta Crystallogr D Biol Crystallogr, 60, 764-766.  
15377221 J.F.Harper, G.Breton, and A.Harmon (2004).
Decoding Ca(2+) signals through plant protein kinases.
  Annu Rev Plant Biol, 55, 263-288.  
14570903 M.Y.Niv, H.Rubin, J.Cohen, L.Tsirulnikov, T.Licht, A.Peretzman-Shemer, E.Cna'an, A.Tartakovsky, I.Stein, S.Albeck, I.Weinstein, M.Goldenberg-Furmanov, D.Tobi, E.Cohen, M.Laster, S.A.Ben-Sasson, and H.Reuveni (2004).
Sequence-based design of kinase inhibitors applicable for therapeutics and target identification.
  J Biol Chem, 279, 1242-1255.  
15229886 N.Fernandez-Fuentes, A.Hermoso, J.Espadaler, E.Querol, F.X.Aviles, and B.Oliva (2004).
Classification of common functional loops of kinase super-families.
  Proteins, 56, 539-555.  
15190080 O.Rey, J.R.Reeve, E.Zhukova, J.Sinnett-Smith, and E.Rozengurt (2004).
G protein-coupled receptor-mediated phosphorylation of the activation loop of protein kinase D: dependence on plasma membrane translocation and protein kinase Cepsilon.
  J Biol Chem, 279, 34361-34372.  
12196540 J.Stamos, M.X.Sliwkowski, and C.Eigenbrot (2002).
Structure of the epidermal growth factor receptor kinase domain alone and in complex with a 4-anilinoquinazoline inhibitor.
  J Biol Chem, 277, 46265-46272.
PDB codes: 1m14 1m17
11525170 O.Mayans, J.Wuerges, S.Canela, M.Gautel, and M.Wilmanns (2001).
Structural evidence for a possible role of reversible disulphide bridge formation in the elasticity of the muscle protein titin.
  Structure, 9, 331-340.
PDB code: 1g1c
11573098 V.Tereshko, M.Teplova, J.Brunzelle, D.M.Watterson, and M.Egli (2001).
Crystal structures of the catalytic domain of human protein kinase associated with apoptosis and tumor suppression.
  Nat Struct Biol, 8, 899-907.
PDB codes: 1ig1 1jkk 1jkl 1jks 1jkt
10629221 S.Hakeda, S.Endo, and K.Saigo (2000).
Requirements of Kettin, a giant muscle protein highly conserved in overall structure in evolution, for normal muscle function, viability, and flight activity of Drosophila.
  J Cell Biol, 148, 101-114.  
9890950 D.A.Enke, P.Kaldis, J.K.Holmes, and M.J.Solomon (1999).
The CDK-activating kinase (Cak1p) from budding yeast has an unusual ATP-binding pocket.
  J Biol Chem, 274, 1949-1956.  
10454192 S.S.Taylor, E.Radzio-Andzelm, Madhusudan, X.Cheng, L.Ten Eyck, and N.Narayana (1999).
Catalytic subunit of cyclic AMP-dependent protein kinase: structure and dynamics of the active site cleft.
  Pharmacol Ther, 82, 133-141.  
9535879 G.Zhi, S.M.Abdullah, and J.T.Stull (1998).
Regulatory segments of Ca2+/calmodulin-dependent protein kinases.
  J Biol Chem, 273, 8951-8957.  
9837816 H.Mehenni, C.Gehrig, J.Nezu, A.Oku, M.Shimane, C.Rossier, N.Guex, J.L.Blouin, H.S.Scott, and S.E.Antonarakis (1998).
Loss of LKB1 kinase activity in Peutz-Jeghers syndrome, and evidence for allelic and locus heterogeneity.
  Am J Hum Genet, 63, 1641-1650.  
9539704 J.Szczepanowska, U.Ramachandran, C.J.Herring, J.M.Gruschus, J.Qin, E.D.Korn, and H.Brzeska (1998).
Effect of mutating the regulatory phosphoserine and conserved threonine on the activity of the expressed catalytic domain of Acanthamoeba myosin I heavy chain kinase.
  Proc Natl Acad Sci U S A, 95, 4146-4151.  
9891791 R.Littlefield, and V.M.Fowler (1998).
Defining actin filament length in striated muscle: rulers and caps or dynamic stability?
  Annu Rev Cell Dev Biol, 14, 487-525.  
9774336 T.A.Millward, C.W.Heizmann, B.W.Schäfer, and B.A.Hemmings (1998).
Calcium regulation of Ndr protein kinase mediated by S100 calcium-binding proteins.
  EMBO J, 17, 5913-5922.  
  9194198 B.Kobe, I.G.Jennings, C.M.House, S.C.Feil, B.J.Michell, T.Tiganis, M.W.Parker, R.G.Cotton, and B.E.Kemp (1997).
Regulation and crystallization of phosphorylated and dephosphorylated forms of truncated dimeric phenylalanine hydroxylase.
  Protein Sci, 6, 1352-1357.  
9312155 D.L.Silver, A.V.Vorotnikov, D.M.Watterson, V.P.Shirinsky, and J.R.Sellers (1997).
Sites of interaction between kinase-related protein and smooth muscle myosin.
  J Biol Chem, 272, 25353-25359.  
9363763 J.Heierhorst, R.J.Mann, and B.E.Kemp (1997).
Interaction of the recombinant S100A1 protein with twitchin kinase, and comparison with other Ca2+-binding proteins.
  Eur J Biochem, 249, 127-133.  
9667861 S.S.Taylor, and E.Radzio-Andzelm (1997).
Protein kinase inhibition: natural and synthetic variations on a theme.
  Curr Opin Chem Biol, 1, 219-226.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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