PDBsum entry 1ha1

Nuclear protein

PDB id

1ha1

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Contents

			Protein chain

					167 a.a. *

			Waters ×170

* Residue conservation analysis

PDB id:

1ha1

Links

Name:

Nuclear protein

Title:

Hnrnp a1 (rbd1,2) from homo sapiens

Structure:

Hnrnp a1. Chain: a. Fragment: hnrnp a1 (rbd1, rbd2). Synonym: heterogeneous nuclear ribonucleoprotein a1, hnrnp a1 1-184. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Cellular_location: nucleus. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.75Å

R-factor:

0.198

R-free:

0.275

Authors:

Y.Shamoo,U.Krueger,L.Rice,K.R.Williams,T.A.Steitz

Key ref:

Y.Shamoo et al. (1997). Crystal structure of the two RNA binding domains of human hnRNP A1 at 1.75 A resolution. Nat Struct Biol, 4, 215-222. PubMed id: 9164463

Date:

30-Oct-96

Release date:

15-May-97

PROCHECK

	Headers

	References

Protein chain

P09651 (ROA1_HUMAN) - Heterogeneous nuclear ribonucleoprotein A1 from Homo sapiens

Seq: Struc:					372 a.a. 167 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Nat Struct Biol 4:215-222 (1997)
PubMed id: 9164463

Crystal structure of the two RNA binding domains of human hnRNP A1 at 1.75 A resolution.
Y.Shamoo, U.Krueger, L.M.Rice, K.R.Williams, T.A.Steitz.

ABSTRACT

Heterogeneous ribonucleoprotein A1 (hnRNP A1) is an abundant eukaryotic nuclear RNA binding protein. A1 is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm and may modulate splice site selection. The crystal structure of A1(RBD1,2) reveals two independently-folded RNA binding domains (RBDs) connected by a flexible linker. Both RBDs are structurally homologous to the U1A(RBD1), and have their RNA binding platforms oriented in an anti-parallel fashion. The anti-parallel arrangement of the A1 RNA binding platforms suggests mechanisms for RNA condensation and ways of bringing together distant RNA sequences for RNA metabolism such as splicing or transport.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20584894

A.Chaudhury, P.Chander, and P.H.Howe (2010).
Heterogeneous nuclear ribonucleoproteins (hnRNPs) in cellular processes: Focus on hnRNP E1's multifunctional regulatory roles.

RNA, 16, 1449-1462.

20080103

C.M.Maynard, and K.B.Hall (2010).
Interactions between PTB RRMs induce slow motions and increase RNA binding affinity.

J Mol Biol, 397, 260-277.

20639884

G.Michlewski, and J.F.Cáceres (2010).
Antagonistic role of hnRNP A1 and KSRP in the regulation of let-7a biogenesis.

Nat Struct Mol Biol, 17, 1011-1018.

19667073

H.L.Okunola, and A.R.Krainer (2009).
Cooperative-binding and splicing-repressive properties of hnRNP A1.

Mol Cell Biol, 29, 5620-5631.

19308324

K.Teilum, J.G.Olsen, and B.B.Kragelund (2009).
Functional aspects of protein flexibility.

Cell Mol Life Sci, 66, 2231-2247.

19718023

M.K.Swan, R.E.Johnson, L.Prakash, S.Prakash, and A.K.Aggarwal (2009).
Structural basis of high-fidelity DNA synthesis by yeast DNA polymerase delta.

Nat Struct Mol Biol, 16, 979-986.

PDB code:

3iay

18201561

A.O.Kumar, M.C.Swenson, M.M.Benning, and C.L.Kielkopf (2008).
Structure of the central RNA recognition motif of human TIA-1 at 1.95A resolution.

Biochem Biophys Res Commun, 367, 813-819.

PDB code:

3bs9

18203745

G.Toba, and K.White (2008).
The third RNA recognition motif of Drosophila ELAV protein has a role in multimerization.

Nucleic Acids Res, 36, 1390-1399.

18953025

T.Nagata, Y.Takada, A.Ono, K.Nagata, Y.Konishi, T.Nukina, M.Ono, A.Matsugami, A.Furukawa, N.Fujimoto, H.Fukuda, H.Nakagama, and M.Katahira (2008).
Elucidation of the mode of interaction in the UP1-telomerase RNA-telomeric DNA ternary complex which serves to recruit telomerase to telomeric DNA and to enhance the telomerase activity.

Nucleic Acids Res, 36, 6816-6824.

17188295

K.R.Thickman, E.A.Sickmier, and C.L.Kielkopf (2007).
Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif.

J Mol Biol, 366, 703-710.

PDB code:

2hzc

17307868

T.Kashima, N.Rao, and J.L.Manley (2007).
An intronic element contributes to splicing repression in spinal muscular atrophy.

Proc Natl Acad Sci U S A, 104, 3426-3431.

16362043

F.Vitali, A.Henning, F.C.Oberstrass, Y.Hargous, S.D.Auweter, M.Erat, and F.H.Allain (2006).
Structure of the two most C-terminal RNA recognition motifs of PTB using segmental isotope labeling.

EMBO J, 25, 150-162.

PDB code:

2evz

15853797

C.Maris, C.Dominguez, and F.H.Allain (2005).
The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression.

FEBS J, 272, 2118-2131.

16094605

V.N.Uversky, C.J.Oldfield, and A.K.Dunker (2005).
Showing your ID: intrinsic disorder as an ID for recognition, regulation and cell signaling.

J Mol Recognit, 18, 343-384.

15004549

C.Alfano, D.Sanfelice, J.Babon, G.Kelly, A.Jacks, S.Curry, and M.R.Conte (2004).
Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein.

Nat Struct Mol Biol, 11, 323-329.

PDB codes:

1s79 1s7a

15520810

R.Schroeder, A.Barta, and K.Semrad (2004).
Strategies for RNA folding and assembly.

Nat Rev Mol Cell Biol, 5, 908-919.

15299008

Y.Iko, T.S.Kodama, N.Kasai, T.Oyama, E.H.Morita, T.Muto, M.Okumura, R.Fujii, T.Takumi, S.Tate, and K.Morikawa (2004).
Domain architectures and characterization of an RNA-binding protein, TLS.

J Biol Chem, 279, 44834-44840.

12842046

A.Jacks, J.Babon, G.Kelly, I.Manolaridis, P.D.Cary, S.Curry, and M.R.Conte (2003).
Structure of the C-terminal domain of human La protein reveals a novel RNA recognition motif coupled to a helical nuclear retention element.

Structure, 11, 833-843.

PDB code:

1owx

12809490

G.C.Pérez-Alvarado, M.Martínez-Yamout, M.M.Allen, R.Grosschedl, H.J.Dyson, and P.E.Wright (2003).
Structure of the nuclear factor ALY: insights into post-transcriptional regulatory and mRNA nuclear export processes.

Biochemistry, 42, 7348-7357.

PDB code:

1no8

12904298

J.C.Myers, S.A.Moore, and Y.Shamoo (2003).
Structure-based incorporation of 6-methyl-8-(2-deoxy-beta-ribofuranosyl)isoxanthopteridine into the human telomeric repeat DNA as a probe for UP1 binding and destabilization of G-tetrad structures.

J Biol Chem, 278, 42300-42306.

PDB codes:

1pgz 1po6

12637517

J.G.De Gaudenzi, I.D'Orso, and A.C.Frasch (2003).
RNA recognition motif-type RNA-binding proteins in Trypanosoma cruzi form a family involved in the interaction with specific transcripts in vivo.

J Biol Chem, 278, 18884-18894.

12773396

J.M.Pérez Cañadillas, and G.Varani (2003).
Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein.

EMBO J, 22, 2821-2830.

PDB code:

1p1t

12738865

M.M.Golas, B.Sander, C.L.Will, R.Lührmann, and H.Stark (2003).
Molecular architecture of the multiprotein splicing factor SF3b.

Science, 300, 980-984.

11917013

J.Vitali, J.Ding, J.Jiang, Y.Zhang, A.R.Krainer, and R.M.Xu (2002).
Correlated alternative side chain conformations in the RNA-recognition motif of heterogeneous nuclear ribonucleoprotein A1.

Nucleic Acids Res, 30, 1531-1538.

PDB code:

1l3k

12388766

P.Björk, G.Baurén, S.Jin, Y.G.Tong, T.R.Bürglin, U.Hellman, and L.Wieslander (2002).
A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis.

Mol Biol Cell, 13, 3683-3695.

12202751

P.Weisman-Shomer, E.Cohen, and M.Fry (2002).
Distinct domains in the CArG-box binding factor A destabilize tetraplex forms of the fragile X expanded sequence d(CGG)n.

Nucleic Acids Res, 30, 3672-3681.

11809873

Z.J.Lorković, and A.Barta (2002).
Genome analysis: RNA recognition motif (RRM) and K homology (KH) domain RNA-binding proteins from the flowering plant Arabidopsis thaliana.

Nucleic Acids Res, 30, 623-635.

11583625

J.D.Schrag, J.J.Bergeron, Y.Li, S.Borisova, M.Hahn, D.Y.Thomas, and M.Cygler (2001).
The Structure of calnexin, an ER chaperone involved in quality control of protein folding.

Mol Cell, 8, 633-644.

PDB code:

1jhn

10679466

A.A.Antson (2000).
Single-stranded-RNA binding proteins.

Curr Opin Struct Biol, 10, 87-94.

10856256

M.R.Conte, T.Grüne, J.Ghuman, G.Kelly, A.Ladas, S.Matthews, and S.Curry (2000).
Structure of tandem RNA recognition motifs from polypyrimidine tract binding protein reveals novel features of the RRM fold.

EMBO J, 19, 3132-3141.

PDB code:

1qm9

10588732

E.Welin Henriksson, M.Wahren-Herlenius, I.Lundberg, E.Mellquist, and I.Pettersson (1999).
Key residues revealed in a major conformational epitope of the U1-70K protein.

Proc Natl Acad Sci U S A, 96, 14487-14492.

10323862

J.Ding, M.K.Hayashi, Y.Zhang, L.Manche, A.R.Krainer, and R.M.Xu (1999).
Crystal structure of the two-RRM domain of hnRNP A1 (UP1) complexed with single-stranded telomeric DNA.

Genes Dev, 13, 1102-1115.

PDB code:

2up1

10542265

J.Gordon, T.K.Sengupta, C.A.Phillips, S.M.O'Malley, K.R.Williams, and E.K.Spicer (1999).
Identification of the RNA binding domain of T4 RegA protein by structure-based mutagenesis.

J Biol Chem, 274, 32265-32273.

9873052

L.A.Dempsey, H.Sun, L.A.Hanakahi, and N.Maizels (1999).
G4 DNA binding by LR1 and its subunits, nucleolin and hnRNP D, A role for G-G pairing in immunoglobulin switch recombination.

J Biol Chem, 274, 1066-1071.

10499800

R.C.Deo, J.B.Bonanno, N.Sonenberg, and S.K.Burley (1999).
Recognition of polyadenylate RNA by the poly(A)-binding protein.

Cell, 98, 835-845.

PDB code:

1cvj

10220389

S.M.Crowder, R.Kanaar, D.C.Rio, and T.Alber (1999).
Absence of interdomain contacts in the crystal structure of the RNA recognition motifs of Sex-lethal.

Proc Natl Acad Sci U S A, 96, 4892-4897.

PDB code:

3sxl

10449418

T.Ito, Y.Muto, M.R.Green, and S.Yokoyama (1999).
Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65)).

EMBO J, 18, 4523-4534.

PDB codes:

1u2f 2u2f

10545321

Y.Zhao, D.Jeruzalmi, I.Moarefi, L.Leighton, R.Lasken, and J.Kuriyan (1999).
Crystal structure of an archaebacterial DNA polymerase.

Structure, 7, 1189-1199.

PDB codes:

1d5a 1qqc

9519296

A.M.Edwards, A.Bochkarev, and L.Frappier (1998).
Origin DNA-binding proteins.

Curr Opin Struct Biol, 8, 49-53.

9740129

A.Mayeda, S.H.Munroe, R.M.Xu, and A.R.Krainer (1998).
Distinct functions of the closely related tandem RNA-recognition motifs of hnRNP A1.

RNA, 4, 1111-1123.

9587002

D.M.Briercheck, T.C.Wood, T.J.Allison, J.P.Richardson, and G.S.Rule (1998).
The NMR structure of the RNA binding domain of E. coli rho factor suggests possible RNA-protein interactions.

Nat Struct Biol, 5, 393-399.

PDB code:

1a63

9646873

G.Varani, and K.Nagai (1998).
RNA recognition by RNP proteins during RNA processing.

Annu Rev Biophys Biomol Struct, 27, 407-445.

9841679

J.E.McCarthy (1998).
Posttranscriptional control of gene expression in yeast.

Microbiol Mol Biol Rev, 62, 1492-1553.

9476892

J.P.Staley, and C.Guthrie (1998).
Mechanical devices of the spliceosome: motors, clocks, springs, and things.

Cell, 92, 315-326.

9848655

K.Zu, M.L.Sikes, and A.L.Beyer (1998).
Separable roles in vivo for the two RNA binding domains of Drosophila A1-hnRNP homolog.

RNA, 4, 1585-1598.

9786934

L.A.Dempsey, L.A.Hanakahi, and N.Maizels (1998).
A specific isoform of hnRNP D interacts with DNA in the LR1 heterodimer: canonical RNA binding motifs in a sequence-specific duplex DNA binding protein.

J Biol Chem, 273, 29224-29229.

9592147

M.Samuels, G.Deshpande, and P.Schedl (1998).
Activities of the Sex-lethal protein in RNA binding and protein:protein interactions.

Nucleic Acids Res, 26, 2625-2637.

9501904

N.Sonenberg, S.K.Burley, and A.C.Gingras (1998).
RNA chiropractics.

Nat Struct Biol, 5, 172-174.

9393863

C.C.Correll, B.Freeborn, P.B.Moore, and T.A.Steitz (1997).
Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain.

Cell, 91, 705-712.

PDB codes:

354d 356d 357d 364d 373d

9311984

P.Bouvet, C.Jain, J.G.Belasco, F.Amalric, and M.Erard (1997).
RNA recognition by the joint action of two nucleolin RNA-binding domains: genetic analysis and structural modeling.

EMBO J, 16, 5235-5246.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.