PDBsum entry 1g2s

Cytokine

PDB id

1g2s

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Contents

			Protein chain

					71 a.a. *

* Residue conservation analysis

PDB id:

1g2s

Links
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Name:

Cytokine

Title:

Solution structure of eotaxin-3

Structure:

Eotaxin-3. Chain: a. Synonym: small inducible cytokine a26, macrophage inflammatory protein 4-alpha. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Organ: lung. Gene: eotaxin-3. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

NMR struc:

1 models

Authors:

J.Ye,K.L.Mayer,M.R.Mayer,M.J.Stone

Key ref:

J.Ye et al. (2001). NMR solution structure and backbone dynamics of the CC chemokine eotaxin-3. Biochemistry, 40, 7820-7831. PubMed id: 11425309 DOI: 10.1021/bi010252s

Date:

20-Oct-00

Release date:

13-Mar-02

PROCHECK

	Headers

	References

Protein chain

Q9Y258 (CCL26_HUMAN) - C-C motif chemokine 26 from Homo sapiens

Seq: Struc:					94 a.a. 71 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

DOI no: 10.1021/bi010252s	Biochemistry 40:7820-7831 (2001)
PubMed id: 11425309

NMR solution structure and backbone dynamics of the CC chemokine eotaxin-3.
J.Ye, K.L.Mayer, M.R.Mayer, M.J.Stone.

ABSTRACT

Eotaxin-3 is one of three related chemokines that specifically activate chemokine receptor CCR3. We report the 3D structure and backbone dynamics of eotaxin-3 determined by NMR spectroscopy. Eotaxin-3 is monomeric under the conditions in this study and consists of an unstructured N-terminus before the first two conserved cysteine residues, an irregularly structured N-loop following the second conserved cysteine, a single turn of 3(10)-helix, a three-stranded antiparallel beta-sheet, an alpha-helix, and an unstructured C-terminal tail. As in other chemokines, the alpha-helix packs against one face of the beta-sheet. The average backbone and heavy atom rmsd values of the 20 structures (residues 9-65) are 0.44 and 1.01 A, respectively. A comparison between the structures of eotaxin-3 and related chemokines suggests that the electrostatic potential in the vicinity of a surface groove and the structure of the beta2-beta3 turn may be important for maintaining receptor specificity. The backbone dynamics of eotaxin-3 were determined from 15N NMR relaxation data using the extended model free dynamics formalism. Large amplitude motions on the picosecond to nanosecond time scale were observed in both termini and in some residues in the N-loop, the beta1-beta2 turn, and the beta3 strand; the location of these residues suggests a possible role for dynamics in receptor binding and activation. In contrast to eotaxin, eotaxin-3 exhibits no substantial mobility on the microsecond to millisecond time scale.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20385598

I.Díaz-Moreno, D.Hollingworth, G.Kelly, S.Martin, M.García-Mayoral, P.Briata, R.Gherzi, and A.Ramos (2010).
Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets.

Nucleic Acids Res, 38, 5193-5205.

19198587

I.Díaz-Moreno, D.Hollingworth, T.A.Frenkiel, G.Kelly, S.Martin, S.Howell, M.García-Mayoral, R.Gherzi, P.Briata, and A.Ramos (2009).
Phosphorylation-mediated unfolding of a KH domain regulates KSRP localization via 14-3-3 binding.

Nat Struct Mol Biol, 16, 238-246.

PDB code:

2opu

19578117

J.Hol, A.M.Küchler, F.E.Johansen, B.Dalhus, G.Haraldsen, and I.Oynebråten (2009).
Molecular requirements for sorting of the chemokine interleukin-8/CXCL8 to endothelial Weibel-Palade bodies.

J Biol Chem, 284, 23532-23539.

18550532

I.V.Nesmelova, Y.Sham, J.Gao, and K.H.Mayo (2008).
CXC and CC Chemokines Form Mixed Heterodimers: ASSOCIATION FREE ENERGIES FROM MOLECULAR DYNAMICS SIMULATIONS AND EXPERIMENTAL CORRELATIONS.

J Biol Chem, 283, 24155-24166.

17635911

E.L.Wise, C.Duchesnes, P.C.da Fonseca, R.A.Allen, T.J.Williams, and J.E.Pease (2007).
Small molecule receptor agonists and antagonists of CCR3 provide insight into mechanisms of chemokine receptor activation.

J Biol Chem, 282, 27935-27943.

17437720

M.F.García-Mayoral, D.Hollingworth, L.Masino, I.Díaz-Moreno, G.Kelly, R.Gherzi, C.F.Chou, C.Y.Chen, and A.Ramos (2007).
The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation.

Structure, 15, 485-498.

PDB codes:

2hh2 2hh3

17075134

O.K.Baryshnikova, and B.D.Sykes (2006).
Backbone dynamics of SDF-1alpha determined by NMR: interpretation in the presence of monomer-dimer equilibrium.

Protein Sci, 15, 2568-2578.

15039444

V.Petkovic, C.Moghini, S.Paoletti, M.Uguccioni, and B.Gerber (2004).
Eotaxin-3/CCL26 is a natural antagonist for CC chemokine receptors 1 and 5. A human chemokine with a regulatory role.

J Biol Chem, 279, 23357-23363.

12486712

K.L.Mayer, and M.J.Stone (2003).
Backbone dynamics of the CC-chemokine eotaxin-2 and comparison among the eotaxin group chemokines.

Proteins, 50, 184-191.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.