spacer
spacer

PDBsum entry 1af9
Go to PDB code: 
protein links
Clostridial neurotoxin PDB id
1af9

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chain
441 a.a. *
Waters ×138
* Residue conservation analysis
PDB id:
1af9
Name: Clostridial neurotoxin
Title: Tetanus neurotoxin c fragment
Structure: Tetanus neurotoxin. Chain: a. Fragment: c fragment. Engineered: yes. Mutation: yes
Source: Clostridium tetani. Organism_taxid: 1513. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: purchased from boehringer mannheim
Resolution:
2.70Å     R-factor:   0.161     R-free:   0.245
Authors: T.C.Umland,L.Wingert,S.Swaminathan,W.F.Furey,J.J.Schmidt,M.Sax
Key ref: T.C.Umland et al. (1997). Structure of the receptor binding fragment HC of tetanus neurotoxin. Nat Struct Biol, 4, 788-792. PubMed id: 9334741
Date:
24-Mar-97     Release date:   29-Apr-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P04958  (TETX_CLOTE) -  Tetanus toxin from Clostridium tetani (strain Massachusetts / E88)
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1315 a.a.
441 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.4.24.68  - tentoxilysin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of 76-Gln-|-Phe-77 bond in synaptobrevin 2.
      Cofactor: Zn(2+)

 

 
Nat Struct Biol 4:788-792 (1997)
PubMed id: 9334741  
 
 
Structure of the receptor binding fragment HC of tetanus neurotoxin.
T.C.Umland, L.M.Wingert, S.Swaminathan, W.F.Furey, J.J.Schmidt, M.Sax.
 
  ABSTRACT  
 
The 2.7 A structure of the tetanus neurotoxin receptor binding fragment Hc reveals a jelly-roll domain and a beta-trefoil domain. Hc retains the unique transport properties of the holotoxin and is capable of eliciting a protective immunological response against the full length holotoxin.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19757026 A.F.Bongat, R.Saksena, R.Adamo, Y.Fujimoto, Z.Shiokawa, D.C.Peterson, K.Fukase, W.F.Vann, and P.Kovác (2010).
Multimeric bivalent immunogens from recombinant tetanus toxin HC fragment, synthetic hexasaccharides, and a glycopeptide adjuvant.
  Glycoconj J, 27, 69-77.  
20219474 P.Stenmark, M.Dong, J.Dupuy, E.R.Chapman, and R.C.Stevens (2010).
Crystal structure of the botulinum neurotoxin type G binding domain: insight into cell surface binding.
  J Mol Biol, 397, 1287-1297.
PDB code: 2vxr
19824793 M.R.Popoff, and P.Bouvet (2009).
Clostridial toxins.
  Future Microbiol, 4, 1021-1064.  
19476346 Z.Fu, C.Chen, J.T.Barbieri, J.J.Kim, and M.R.Baldwin (2009).
Glycosylated SV2 and gangliosides as dual receptors for botulinum neurotoxin serotype F.
  Biochemistry, 48, 5631-5641.
PDB codes: 3fuo 3fuq
17185412 A.Rummel, T.Eichner, T.Weil, T.Karnath, A.Gutcaits, S.Mahrhold, K.Sandhoff, R.L.Proia, K.R.Acharya, H.Bigalke, and T.Binz (2007).
Identification of the protein receptor binding site of botulinum neurotoxins B and G proves the double-receptor concept.
  Proc Natl Acad Sci U S A, 104, 359-364.  
17916047 P.Chiarella, E.Massi, M.De Robertis, E.Signori, and V.M.Fazio (2007).
Adjuvants in vaccines and for immunisation: current trends.
  Expert Opin Biol Ther, 7, 1551-1562.  
17200883 T.Suzuki, H.Kouguchi, T.Watanabe, K.Hasegawa, T.Yoneyama, K.Niwa, A.Nishikawa, J.C.Lee, K.Oguma, and T.Ohyama (2007).
Effect of nicking the C-terminal region of the Clostridium botulinum serotype D neurotoxin heavy chain on its toxicity and molecular properties.
  Protein J, 26, 173-181.  
16861677 O.Qazi, D.Sesardic, R.Tierney, Z.Söderbäck, D.Crane, B.Bolgiano, and N.Fairweather (2006).
Reduction of the ganglioside binding activity of the tetanus toxin HC fragment destroys immunogenicity: implications for development of novel tetanus vaccines.
  Infect Immun, 74, 4884-4891.  
15749706 C.X.Moss, S.P.Matthews, D.J.Lamont, and C.Watts (2005).
Asparagine deamidation perturbs antigen presentation on class II major histocompatibility complex molecules.
  J Biol Chem, 280, 18498-18503.  
16104015 S.Jayaraman, S.Eswaramoorthy, D.Kumaran, and S.Swaminathan (2005).
Common binding site for disialyllactose and tri-peptide in C-fragment of tetanus neurotoxin.
  Proteins, 61, 288-295.
PDB codes: 1yxw 1yyn
14731268 A.Rummel, S.Mahrhold, H.Bigalke, and T.Binz (2004).
The HCC-domain of botulinum neurotoxins A and B exhibits a singular ganglioside binding site displaying serotype specific carbohydrate interaction.
  Mol Microbiol, 51, 631-643.  
15381192 C.Montecucco, O.Rossetto, and G.Schiavo (2004).
Presynaptic receptor arrays for clostridial neurotoxins.
  Trends Microbiol, 12, 442-446.  
15292504 F.K.Stevenson, C.H.Ottensmeier, P.Johnson, D.Zhu, S.L.Buchan, K.J.McCann, J.S.Roddick, A.T.King, F.McNicholl, N.Savelyeva, and J.Rice (2004).
DNA vaccines to attack cancer.
  Proc Natl Acad Sci U S A, 101, 14646-14652.  
15233733 F.K.Stevenson, J.Rice, C.H.Ottensmeier, S.M.Thirdborough, and D.Zhu (2004).
DNA fusion gene vaccines against cancer: from the laboratory to the clinic.
  Immunol Rev, 199, 156-180.  
15500391 K.A.Foster (2004).
The analgesic potential of clostridial neurotoxin derivatives.
  Expert Opin Investig Drugs, 13, 1437-1443.  
14744243 L.L.Simpson (2004).
Identification of the major steps in botulinum toxin action.
  Annu Rev Pharmacol Toxicol, 44, 167-193.  
13678859 G.Lalli, S.Bohnert, K.Deinhardt, C.Verastegui, and G.Schiavo (2003).
The journey of tetanus and botulinum neurotoxins in neurons.
  Trends Microbiol, 11, 431-437.  
12911593 M.T.Reding, D.K.Okita, B.M.Diethelm-Okita, T.A.Anderson, and B.M.Conti-Fine (2003).
Human CD4+ T-cell epitope repertoire on the C2 domain of coagulation factor VIII.
  J Thromb Haemost, 1, 1777-1784.  
12417130 K.Turton, J.A.Chaddock, and K.R.Acharya (2002).
Botulinum and tetanus neurotoxins: structure, function and therapeutic utility.
  Trends Biochem Sci, 27, 552-558.  
11119555 D.D.Pless, E.R.Torres, E.K.Reinke, and S.Bavari (2001).
High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A.
  Infect Immun, 69, 570-574.  
11501761 V.I.Rotari, P.M.Dando, and A.J.Barrett (2001).
Legumain forms from plants and animals differ in their specificity.
  Biol Chem, 382, 953-959.  
10795737 A.N.Antoniou, S.L.Blackwood, D.Mazzeo, and C.Watts (2000).
Control of antigen presentation by a single protease cleavage site.
  Immunity, 12, 391-398.  
10945801 J.J.Farrar, L.M.Yen, T.Cook, N.Fairweather, N.Binh, J.Parry, and C.M.Parry (2000).
Tetanus.
  J Neurol Neurosurg Psychiatry, 69, 292-301.  
10972823 K.Sinha, M.Box, G.Lalli, G.Schiavo, H.Schneider, M.Groves, G.Siligardi, and N.Fairweather (2000).
Analysis of mutants of tetanus toxin Hc fragment: ganglioside binding, cell binding and retrograde axonal transport properties.
  Mol Microbiol, 37, 1041-1051.  
11086225 M.P.Byrne, and L.A.Smith (2000).
Development of vaccines for prevention of botulism.
  Biochimie, 82, 955-966.  
10722735 P.Emsley, C.Fotinou, I.Black, N.F.Fairweather, I.G.Charles, C.Watts, E.Hewitt, and N.W.Isaacs (2000).
The structures of the H(C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding.
  J Biol Chem, 275, 8889-8894.
PDB codes: 1d0h 1dfq 1diw 1dll
10547701 E.A.Johnson (1999).
Clostridial toxins as therapeutic agents: benefits of nature's most toxic proteins.
  Annu Rev Microbiol, 53, 551-575.  
10520997 G.Rudenko, T.Nguyen, Y.Chelliah, T.C.Südhof, and J.Deisenhofer (1999).
The structure of the ligand-binding domain of neurexin Ibeta: regulation of LNS domain function by alternative splicing.
  Cell, 99, 93.
PDB code: 1c4r
10455200 L.C.Williamson, K.E.Bateman, J.C.Clifford, and E.A.Neale (1999).
Neuronal sensitivity to tetanus toxin requires gangliosides.
  J Biol Chem, 274, 25173-25180.  
10212474 R.Pellizzari, O.Rossetto, G.Schiavo, and C.Montecucco (1999).
Tetanus and botulinum neurotoxins: mechanism of action and therapeutic uses.
  Philos Trans R Soc Lond B Biol Sci, 354, 259-268.  
9795759 C.Watts, A.Antoniou, B.Manoury, E.W.Hewitt, L.M.Mckay, L.Grayson, N.F.Fairweather, P.Emsley, N.Isaacs, and P.D.Simitsek (1998).
Modulation by epitope-specific antibodies of class II MHC-restricted presentation of the tetanus toxin antigen.
  Immunol Rev, 164, 11-16.  
9914258 D.B.Lacy, and R.C.Stevens (1998).
Unraveling the structures and modes of action of bacterial toxins.
  Curr Opin Struct Biol, 8, 778-784.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

spacer
spacer