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PDBsum entry 1ad2
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protein ligands metals links
Ribosomal protein PDB id
1ad2

 

 

 

 

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Contents
Protein chain
224 a.a. *
Ligands
SO4 ×3
MRD
Metals
_HG
Waters ×122
* Residue conservation analysis
PDB id:
1ad2
Name: Ribosomal protein
Title: Ribosomal protein l1 mutant with serine 179 replaced by cysteine
Structure: Ribosomal protein l1. Chain: a. Synonym: tl2. Engineered: yes. Mutation: yes
Source: Thermus thermophilus. Organism_taxid: 274. Strain: vk-1. Organelle: ribosome. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Resolution:
1.90Å     R-factor:   0.203     R-free:   0.270
Authors: J.Unge,S.Al-Karadaghi,A.Liljas,B.-H.Jonsson,I.Eliseikina,N.Ossina, N.Nevskaya,N.Fomenkova,M.Garber,S.Nikonov
Key ref: J.Unge et al. (1997). A mutant form of the ribosomal protein L1 reveals conformational flexibility. Febs Lett, 411, 53-59. PubMed id: 9247141
Date:
20-Feb-97     Release date:   15-May-97    
PROCHECK
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 Headers
 References

Protein chain
P27150  (RL1_THETH) -  Large ribosomal subunit protein uL1 from Thermus thermophilus
Seq:
Struc: 		229 a.a.
224 a.a.*
Key:    Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 

 
Febs Lett 411:53-59 (1997)
PubMed id: 9247141  
 
 
A mutant form of the ribosomal protein L1 reveals conformational flexibility.
J.Unge, S.Al-Karadaghi, A.Liljas, B.H.Jonsson, I.Eliseikina, N.Ossina, N.Nevskaya, N.Fomenkova, M.Garber, S.Nikonov.
 
  ABSTRACT  
 
The crystal structure of the mutant S179C of the ribosomal protein L1 from Thermus thermophilus has been determined at 1.9 A resolution. The mutant molecule displays a small but significant opening of the cavity between the two domains. The domain movement seems to be facilitated by the flexibility of at least two conserved glycines. These glycines may be necessary for the larger conformational change needed for an induced fit mechanism upon binding RNA. The domain movement makes a disulfide bridge possible between the incorporated cysteines in two monomers of the mutant L1.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
12037305 N.Nevskaya, S.Tishchenko, M.Paveliev, Y.Smolinskaya, R.Fedorov, W.Piendl, Y.Nakamura, T.Toyoda, M.Garber, and S.Nikonov (2002).
Structure of ribosomal protein L1 from Methanococcus thermolithotrophicus. Functionally important structural invariants on the L1 surface.
  Acta Crystallogr D Biol Crystallogr, 58, 1023-1029.
PDB code: 1dwu
10799526 D.I.Svergun, and K.H.Nierhaus (2000).
A map of protein-rRNA distribution in the 70 S Escherichia coli ribosome.
  J Biol Chem, 275, 14432-14439.  
10801481 N.Nevskaya, S.Tischenko, R.Fedorov, S.Al-Karadaghi, A.Liljas, A.Kraft, W.Piendl, M.Garber, and S.Nikonov (2000).
Archaeal ribosomal protein L1: the structure provides new insights into RNA binding of the L1 protein family.
  Structure, 8, 363-371.
PDB code: 1cjs
10531479 R.Fedorov, N.Nevskaya, A.Khairullina, S.Tishchenko, A.Mikhailov, M.Garber, and S.Nikonov (1999).
Structure of ribosomal protein L30 from Thermus thermophilus at 1.9 A resolution: conformational flexibility of the molecule.
  Acta Crystallogr D Biol Crystallogr, 55, 1827-1833.
PDB code: 1bxy
  9705143 S.V.Nikonov, N.A.Nevskaya, R.V.Fedorov, A.R.Khairullina, S.V.Tishchenko, A.D.Nikulin, and M.B.Garber (1998).
Structural studies of ribosomal proteins.
  Biol Chem, 379, 795-805.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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