PDBsum entry 1dm7

Oxidoreductase

PDB id: 1dm7

Contents

Protein chains

416 a.a. *

Ligands

ACT ×2

CAC ×2

HEM ×2

HRG ×2

GOL ×5

Metals

_ZN

Waters ×409

* Residue conservation analysis

PDB id:

1dm7

Name:

Oxidoreductase

Title:

Bovine endothelial nitric oxide synthase heme domain complexed with homoarginine (h4b free)

Structure:

Nitric oxide synthase. Chain: a, b. Fragment: heme domain. Engineered: yes

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Cell: endothelial. Expressed in: escherichia coli. Expression_system_taxid: 562

Biol. unit:

Dimer (from PQS)

Resolution:

2.10Å R-factor: 0.231 R-free: 0.273

Authors:

C.S.Raman,H.Li,P.Martasek,G.J.Southan,B.S.S.Masters,T.L.Poulos

Key ref:

C.S.Raman et al. Crystal structures of bovine endothelial nitric oxide synthase heme domain complexed with various inhibitors. To be published, .

Date:

13-Dec-99 Release date: 13-Dec-00

Protein chains

P29473  (NOS3_BOVIN) -  Nitric oxide synthase 3 from Bos taurus

Seq: 1205 a.a.

Struc: 416 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.14.13.39 - nitric-oxide synthase (NADPH).
• Reaction: 2 L-arginine + 3 NADPH + 4 O2 + H⁺ = 2 L-citrulline + 2 nitric oxide + 3 NADP⁺ + 4 H2O

2 × L-arginine + 3 × NADPH (Bound ligand (Het Group name = HRG) matches with 56.25% similarity) + 4 × O2 + H(+) = 2 × L-citrulline + 2 × nitric oxide + 3 × NADP(+) + 4 × H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site