› 8ya7

X-ray diffraction
1.99Å resolution

endo-1,3-fucanase Fun168A,complex with fucotetraose

Released:
DOI: 10.2210/pdb8ya7/pdb
PDB entry 8ya7 coloured by chain, front view.
PDB entry 8ya7 coloured by chain, side view.
PDB entry 8ya7 coloured by chain, top view.
Source organism: Wenyingzhuangia fucanilytica
Entry authors: Chen GN, Chang YG

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
endo-1,3-fucanase Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 48.31 KDa
Source organism: Wenyingzhuangia fucanilytica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1B1Y779 (Residues: 19-408; Coverage: 100%)
Gene name: AXE80_10060
Sequence domains: Hypothetical glycosyl hydrolase family 15

Ligands and Environments

Carbohydrate polymer : NEW Components: X6Y, X2Y, FUC
Ligand X6Y 2 x X6Y
Ligand X2Y 1 x X2Y
Ligand FUC 1 x FUC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 54.017Å b: 67.29Å c: 239.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.195 0.254
Expression system: Escherichia coli BL21(DE3)

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