› 8v4h

X-ray diffraction
2.2Å resolution

X-ray structure of the NADP-dependent reductase from Campylobacter jejuni responsible for the synthesis of CDP-glucitol in the presence of CDP-glucitol

Released:
DOI: 10.2210/pdb8v4h/pdb
PDB entry 8v4h coloured by chain, front view.
PDB entry 8v4h coloured by chain, side view.
PDB entry 8v4h coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Biosynthesis of Cytidine Diphosphate-6-d-Glucitol for the Capsular Polysaccharides of Campylobacter jejuni.
Ghosh MK, Narindoshvili T, Thoden JB, Schumann ME, Holden HM, Raushel FM
Biochemistry (2024)
PMID: 38386885

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDP-6-D-glucitol synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 41.09 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0U3AP28 (Residues: 1-335; Coverage: 100%)
Gene name: HS5.17
Sequence domains: NAD dependent epimerase/dehydratase family

Ligands and Environments

4 bound ligands:
Ligand YCX 2 x YCX
Ligand CL 2 x CL
Ligand PO4 1 x PO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P212121
Unit cell:
a: 54.76Å b: 101.593Å c: 133.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.207 0.282
Expression system: Escherichia coli BL21(DE3)

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