› 8pmi

X-ray diffraction
2.83Å resolution

Structure of Nal1 indica cultivar IR64, construct 36-458 in presence of peptide from FZP protein

Released:
DOI: 10.2210/pdb8pmi/pdb
PDB entry 8pmi coloured by chain, front view.
PDB entry 8pmi coloured by chain, side view.
PDB entry 8pmi coloured by chain, top view.
Source organism: Oryza sativa Indica Group
Primary publication:
The catalytic triad of rice NARROW LEAF1 involves H234.
Huang LY, Liu NN, Chen WF, Ai X, Li HH, Zhang ZL, Hou XM, Fossé P, Mauffret O, Lei DS, Rety S, Xi XG
Nat Plants (2024)
PMID: 38600265

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein NARROW LEAF 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 423 amino acids
Theoretical weight: 46.21 KDa
Source organism: Oryza sativa Indica Group
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: B4XT64 (Residues: 36-458; Coverage: 73%)
Gene names: GFP, LOC_Os04g52479, LSCHL4, NAL1, Os04g0615000, OsJ_16147, SPIKE

Ligands and Environments

4 bound ligands:
Ligand ATP 6 x ATP
Ligand MG 7 x MG
Ligand SO4 12 x SO4
Ligand NH4 1 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 174.276Å b: 189.186Å c: 89.922Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.268
Expression system: Escherichia coli K-12

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