8pei

X-ray diffraction
3Å resolution

Crystal structure of the biphotochromic fluorescent protein SAASoti (C21N/V127T variant) in its green on-state

Released:
Source organism: Stylocoeniella armata
Entry authors: Boyko KM, Varfolomeeva LA, Matyuta IO, Gavshina AV, Solovyev ID, Popov VO, Savitsky AP

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-238966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C21N/V127T form of the biphotochromic fluorescent protein SAASoti Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 26.26 KDa
Source organism: Stylocoeniella armata
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6122
Unit cell:
a: 104.583Å b: 104.583Å c: 151.187Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.294 0.291 0.34
Expression system: Escherichia coli BL21(DE3)