› 8ow7

X-ray diffraction
3.06Å resolution

Crystal structure of Tannerella forsythia sugar kinase K1058 in complex with N-acetylmuramic acid (MurNAc)

Released:
DOI: 10.2210/pdb8ow7/pdb
PDB entry 8ow7 coloured by chain, front view.
PDB entry 8ow7 coloured by chain, side view.
PDB entry 8ow7 coloured by chain, top view.
Source organism: Tannerella forsythia
Primary publication:
N-acetylmuramic acid recognition by MurK kinase from the MurNAc auxotrophic oral pathogen Tannerella forsythia.
Stasiak AC, Gogler K, Borisova M, Fink P, Mayer C, Stehle T, Zocher G
J Biol Chem 105076 (2023)
PMID: 37481208

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BadF/BadG/BcrA/BcrD ATPase family protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 293 amino acids
Theoretical weight: 33.04 KDa
Source organism: Tannerella forsythia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G8UQH1 (Residues: 20-302; Coverage: 94%)
Gene name: BFO_0034

Ligands and Environments

2 bound ligands:
Ligand AMU 6 x AMU
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 143.75Å b: 143.75Å c: 210.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.219 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

N-acetylmuramic acid recognition by MurK kinase from the MurNAc auxotrophic oral pathogen Tannerella forsythia.
Stasiak et al. (2023)