› 8oha

X-ray diffraction
2.37Å resolution

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-227460 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D, F, G, H
Molecule details ›
Chains: A, B, C, D, F, G, H
Length: 337 amino acids
Theoretical weight: 36.86 KDa
Source organism: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72QM3 (Residues: 1-335; Coverage: 100%)
Gene names: LIC_12090, gapA
Sequence domains:
Glyceraldehyde-3-phosphate dehydrogenase Chain: E
Molecule details ›
Chain: E
Length: 337 amino acids
Theoretical weight: 36.82 KDa
Source organism: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72QM3 (Residues: 1-335; Coverage: 100%)
Gene names: LIC_12090, gapA
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
3 bound ligands:
Ligand PEG 7 x PEG
Ligand GOL 33 x GOL
Ligand PO4 4 x PO4
1 modified residue:
Ligand CSD 7 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P1
Unit cell:
a: 79.804Å b: 82.039Å c: 123.182Å
α: 94.02° β: 95.15° γ: 112.52°
R-values:
R R work R free
0.192 0.191 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

The structure of Leptospira interrogans GAPDH sheds light into an immunoevasion factor that can target the anaphylatoxin C5a of innate immunity.
Navas-Yuste et al. (2023)