› 8ixm

X-ray diffraction
1.96Å resolution

Pseudomoans Aerugiona Wildtype Ketopantoate Reductase ternary complex with NADP+ and alpha-Ketoisocaproic acid

Released:
DOI: 10.2210/pdb8ixm/pdb
PDB entry 8ixm coloured by chain, front view.
PDB entry 8ixm coloured by chain, side view.
PDB entry 8ixm coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Choudhury GB, Datta S

Function and Biology Details

Reaction catalysed: 
(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydropantoate 2-reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.21 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I2Y5 (Residues: 1-315; Coverage: 100%)
Gene name: PA1752
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
3 bound ligands:
Ligand NHE 1 x NHE
Ligand GOL 1 x GOL
Ligand COI 2 x COI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER X8 PROTEUM
Spacegroup: P21
Unit cell:
a: 66.587Å b: 46.769Å c: 95.775Å
α: 90° β: 98.11° γ: 90°
R-values:
R R work R free
0.194 0.192 0.238
Expression system: Escherichia coli BL21

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