› 8ixi

X-ray diffraction
2.28Å resolution

Crystal structure of aldehyde dehydrogenase (EC 1.2.1.3) Klebsiella pneumoniae

Released:
DOI: 10.2210/pdb8ixi/pdb
PDB entry 8ixi coloured by chain, front view.
PDB entry 8ixi coloured by chain, side view.
PDB entry 8ixi coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Systems engineering of Escherichia coli for high-level glutarate production from glucose.
Zhang Z, Chu R, Wei W, Song W, Ye C, Chen X, Wu J, Liu L, Gao C
Nat Commun 15 1032 (2024)
PMID: 38310110

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 33.85 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: D8L2F8 (Residues: 1-294; Coverage: 100%)
Gene names: G4V31_28655, IE995_27805, NCTC13443_00027, mph(E), mph2, mphE
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P21
Unit cell:
a: 53.447Å b: 89.027Å c: 65.997Å
α: 90° β: 93.95° γ: 90°
R-values:
R R work R free
0.213 0.211 0.264
Expression system: Escherichia coli

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