› 8hja

X-ray diffraction
2.38Å resolution

The crystal structure of syn_CdgR-(c-di-GMP) from Synechocystis sp. PCC 6803

Released:
DOI: 10.2210/pdb8hja/pdb
PDB entry 8hja coloured by chain, front view.
PDB entry 8hja coloured by chain, side view.
PDB entry 8hja coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
A c-di-GMP binding effector controls cell size in a cyanobacterium.
Zeng X, Huang M, Sun QX, Peng YJ, Xu X, Tang YB, Zhang JY, Yang Y, Zhang CC
Proc Natl Acad Sci U S A 120 e2221874120 (2023)
PMID: 36947515

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Competence protein ComFB Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 20.63 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P74113 (Residues: 1-173; Coverage: 100%)
Gene name: slr1970
Sequence domains: Late competence development protein ComFB

Ligands and Environments

1 bound ligand:
Ligand C2E 2 x C2E
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 96.327Å b: 55.978Å c: 91.692Å
α: 90° β: 111.97° γ: 90°
R-values:
R R work R free
0.231 0.224 0.286
Expression system: Escherichia coli

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Citations

3 review citations

Control of light-dependent behaviour in cyanobacteria by the second messenger cyclic di-GMP.
Enomoto et al. (2023)
2 more