› 8h4j

X-ray diffraction
2Å resolution

Crystal Structure of AzoR-FMN-Lyb24 complex

Released:
DOI: 10.2210/pdb8h4j/pdb
PDB entry 8h4j coloured by chain, front view.
PDB entry 8h4j coloured by chain, side view.
PDB entry 8h4j coloured by chain, top view.
Source organism: Klebsiella pneumoniae 30684/NJST258_2
Entry author: Huang W

Function and Biology Details

Reaction catalysed: 
Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FMN dependent NADH:quinone oxidoreductase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 210 amino acids
Theoretical weight: 22.78 KDa
Source organism: Klebsiella pneumoniae 30684/NJST258_2
Expression system: Escherichia coli
UniProt:
  • Canonical: W8UQZ7 (Residues: 2-201; Coverage: 100%)
Gene names: KPNJ2_01278, azoR
Sequence domains: Flavodoxin-like fold

Ligands and Environments

2 bound ligands:
Ligand FMN 8 x FMN
Ligand 1IE 8 x 1IE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: F222
Unit cell:
a: 143.04Å b: 160.11Å c: 335.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.211
Expression system: Escherichia coli

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