› 8gyb

X-ray diffraction
2.1Å resolution

Crystal structure of Alongshan virus methyltransferase bound to S-adenosyl-L-homocysteine

Released:
DOI: 10.2210/pdb8gyb/pdb
PDB entry 8gyb coloured by chain, front view.
PDB entry 8gyb coloured by chain, side view.
PDB entry 8gyb coloured by chain, top view.
Source organism: Alongshan virus
Primary publication:
Structural and functional basis of low-affinity SAM/SAH-binding in the conserved MTase of the multi-segmented Alongshan virus distantly related to canonical unsegmented flaviviruses.
Chen H, Lin S, Yang F, Chen Z, Guo L, Yang J, Lin X, Wang L, Duan Y, Wen A, Zhang X, Dai Y, Yin K, Yuan X, Yu C, He Y, He B, Cao Y, Dong H, Li J, Zhao Q, Liu Q, Lu G
PLoS Pathog 19 e1011694 (2023)
PMID: 37831643

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RdRp catalytic domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 277 amino acids
Theoretical weight: 31.86 KDa
Source organism: Alongshan virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A344X2I6 (Residues: 42-313; Coverage: 30%)
Sequence domains: FtsJ-like methyltransferase

Ligands and Environments

1 bound ligand:
Ligand SAH 4 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2221
Unit cell:
a: 90.841Å b: 170.508Å c: 172.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.236
Expression system: Escherichia coli BL21(DE3)

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