› 8g12

Electron Microscopy
2.17Å resolution

CryoEM structure of nuclear GAPDH under 8h Oxidative Stress

Released:
DOI: 10.2210/pdb8g12/pdb
PDB entry 8g12 coloured by chain, front view.
PDB entry 8g12 coloured by chain, side view.
PDB entry 8g12 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Efficient tagging of endogenous proteins in human cell lines for structural studies by single-particle cryo-EM.
Choi W, Wu H, Yserentant K, Huang B, Cheng Y
Proc Natl Acad Sci U S A 120 e2302471120 (2023)
PMID: 37487103
Related structures: EMD-29659

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 334 amino acids
Theoretical weight: 35.98 KDa
Source organism: Homo sapiens
Expression system: Homo
UniProt:
  • Canonical: P04406 (Residues: 2-335; Coverage: 100%)
Gene names: CDABP0047, GAPD, GAPDH, OK/SW-cl.12
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSO 4 x CSO

Experiments and Validation Details

Entry percentile scores
Resolution: 2.17Å
Relevant EMDB volumes: EMD-29659
Expression system: Homo

Quick links

Citations

1 mention without citation

Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody.
Gu et al. (2015)