› 8fmh

X-ray diffraction
1.87Å resolution

Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-dGAMP ligand (2 tetramers in the AU)

Released:
DOI: 10.2210/pdb8fmh/pdb
PDB entry 8fmh coloured by chain, front view.
PDB entry 8fmh coloured by chain, side view.
PDB entry 8fmh coloured by chain, top view.
Source organism: Pseudomonas syringae
Primary publication:
Activation of CBASS Cap5 endonuclease immune effector by cyclic nucleotides.
Rechkoblit O, Sciaky D, Kreitler DF, Buku A, Kottur J, Aggarwal AK
Nat Struct Mol Biol (2024)
PMID: 38321146

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HNH endonuclease Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 388 amino acids
Theoretical weight: 42.77 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2P0QGK5 (Residues: 10-397; Coverage: 98%)
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand 4UR 8 x 4UR
Ligand ZN 8 x ZN
Ligand MG 10 x MG
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21
Unit cell:
a: 67.97Å b: 295.678Å c: 84.052Å
α: 90° β: 113.79° γ: 90°
R-values:
R R work R free
0.212 0.21 0.253
Expression system: Escherichia coli

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