› 8dfa

Electron Microscopy
2.8Å resolution

type I-C Cascade bound to ssDNA target

Released:
DOI: 10.2210/pdb8dfa/pdb
PDB entry 8dfa coloured by chain, front view.
PDB entry 8dfa coloured by chain, side view.
PDB entry 8dfa coloured by chain, top view.
Source organisms:
Primary publication:
Structural snapshots of R-loop formation by a type I-C CRISPR Cascade.
O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW
Mol Cell (2023)
PMID: 36805026
Related structures: EMD-27403

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-113278 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (6 distinct):
pre-crRNA processing endonuclease Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.98 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
Expression system: Escherichia coli
CRISPR-associated protein, TM1801 family Chains: B, C, D, E, F, G, H
Molecule details ›
Chains: B, C, D, E, F, G, H
Length: 290 amino acids
Theoretical weight: 32.36 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
Expression system: Escherichia coli
CRISPR-associated protein, CT1133 family Chain: I
Molecule details ›
Chain: I
Length: 612 amino acids
Theoretical weight: 68.12 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
Expression system: Escherichia coli
CRISPR-associated protein, CT1133 family Chains: J, K
Molecule details ›
Chains: J, K
Length: 124 amino acids
Theoretical weight: 14.02 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
Expression system: Escherichia coli
RNA (46-MER) Chain: L
Molecule details ›
Chain: L
Length: 46 nucleotides
Theoretical weight: 14.82 KDa
Source organism: Desulfovibrio vulgaris
Expression system: Escherichia coli
TS Chain: N
Molecule details ›
Chain: N
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-27403
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Helicases in R-loop Formation and Resolution.
Yang et al. (2023)
2 other articles

1 mention without citation

Structural snapshots of R-loop formation by a type I-C CRISPR Cascade.
O'Brien et al. (2023)