› 8d9p

X-ray diffraction
1.9Å resolution

De Novo Photosynthetic Reaction Center Protein Equipped with Heme B and Mn(II) cations

Released:
DOI: 10.2210/pdb8d9p/pdb
PDB entry 8d9p coloured by chain, front view.
PDB entry 8d9p coloured by chain, side view.
PDB entry 8d9p coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Rational design of photosynthetic reaction center protein maquettes.
Ennist NM, Stayrook SE, Dutton PL, Moser CC
Front Mol Biosci 9 997295 (2022)
PMID: 36213121

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reaction center maquette Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.53 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand MN 2 x MN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41212
Unit cell:
a: 44.968Å b: 44.968Å c: 238.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.243
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Design of Heme Enzymes with a Tunable Substrate Binding Pocket Adjacent to an Open Metal Coordination Site.
Kalvet et al. (2023)
2 more

1 mention without citation

Rational design of photosynthetic reaction center protein maquettes.
Ennist et al. (2022)