› 8d0r

X-ray diffraction
1.4Å resolution

Human FUT9 bound to GDP and H-Type 2

Released:
DOI: 10.2210/pdb8d0r/pdb
PDB entry 8d0r coloured by chain, front view.
PDB entry 8d0r coloured by chain, side view.
PDB entry 8d0r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9.
Kadirvelraj R, Boruah BM, Wang S, Chapla D, Huang C, Ramiah A, Hudson KL, Prudden AR, Boons GJ, Withers SG, Wood ZA, Moremen KW
Nat Chem Biol (2023)
PMID: 37202521

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->4)-(alpha-L-fucosyl-(1->3))-N-acetyl-D-glucosaminyl-R
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 37.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y231 (Residues: 39-359; Coverage: 89%)
Gene name: FUT9
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, FUC
Carbohydrate polymer
3 bound ligands:
Ligand GDP 1 x GDP
Ligand SO4 3 x SO4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I213
Unit cell:
a: 127.69Å b: 127.69Å c: 127.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.168
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Direct methylation sequencing.
Abakir et al. (2023)