› 8cxs

X-ray diffraction
2.49Å resolution

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222869 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
site-specific DNA-methyltransferase (adenine-specific) Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 578 amino acids
Theoretical weight: 68.89 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q183J3 (Residues: 1-577; Coverage: 100%)
Gene name: CD630_27580
Sequence domains:
DNA Strand 2 Chains: E, G, I
Molecule details ›
Chains: E, G, I
Length: 14 nucleotides
Theoretical weight: 4.33 KDa
Source organism: Clostridioides difficile
Expression system: Not provided
DNA Strand 1 Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 14 nucleotides
Theoretical weight: 4.23 KDa
Source organism: Clostridioides difficile
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand K 5 x K
Ligand EDO 11 x EDO
Ligand MTA 3 x MTA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 81.97Å b: 161.159Å c: 230.795Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.213
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 citation in other articles

Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the bdelloid rotifer Adineta vaga.
Zhou et al. (2023)
1 more

1 mention without citation

Systematic Design of Adenosine Analogs as Inhibitors of a Clostridioides difficile-Specific DNA Adenine Methyltransferase Required for Normal Sporulation and Persistence.
Zhou et al. (2023)