› 8c6k

X-ray diffraction
2.86Å resolution

Double mutant A(L53)C/I(L64)C structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV

Released:
DOI: 10.2210/pdb8c6k/pdb
PDB entry 8c6k coloured by chain, front view.
PDB entry 8c6k coloured by chain, side view.
PDB entry 8c6k coloured by chain, top view.
Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
Stabilization of Cereibacter sphaeroides Photosynthetic Reaction Center by the Introduction of Disulfide Bonds.
Selikhanov G, Atamas A, Yukhimchuk D, Fufina T, Vasilieva L, Gabdulkhakov A
Membranes (Basel) 13 (2023)
PMID: 36837657

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174601 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 242 amino acids
Theoretical weight: 26.28 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3J170 (Residues: 9-250; Coverage: 93%)
Gene names: RHOS4_18960, RSP_0291, puhA
Sequence domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.38 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3J1A5 (Residues: 2-282; Coverage: 100%)
Gene names: RHOS4_18610, RSP_0257, pufL
Sequence domains: Photosynthetic reaction centre protein
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 303 amino acids
Theoretical weight: 34.02 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3J1A6 (Residues: 2-304; Coverage: 98%)
Gene names: RHOS4_18600, RSP_0256, pufM
Sequence domains: Photosynthetic reaction centre protein

Ligands and Environments

13 bound ligands:
Ligand LDA 6 x LDA
Ligand UNL 10 x UNL
Ligand PO4 3 x PO4
Ligand EDO 7 x EDO
Ligand K 1 x K
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand U10 2 x U10
Ligand DIO 1 x DIO
Ligand HTO 2 x HTO
Ligand CDL 1 x CDL
Ligand FE 1 x FE
Ligand SPN 1 x SPN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P3121
Unit cell:
a: 139.636Å b: 139.636Å c: 185.091Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.228 0.279
Expression system: Cereibacter sphaeroides 2.4.1

Quick links

Citations

1 citations in other articles

Membrane Proteins: Function, Structure, and Dynamics.
Senju et al. (2023)