› 8c2o

X-ray diffraction
2.35Å resolution

Structure of E. coli AmiA

Released:
DOI: 10.2210/pdb8c2o/pdb
PDB entry 8c2o coloured by chain, front view.
PDB entry 8c2o coloured by chain, side view.
PDB entry 8c2o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Activator-induced conformational changes regulate division-associated peptidoglycan amidases.
Cook J, Baverstock TC, McAndrew MBL, Roper DI, Stansfeld PJ, Crow A
Proc Natl Acad Sci U S A 120 e2302580120 (2023)
PMID: 37276423

Function and Biology Details

Reaction catalysed: 
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylmuramoyl-L-alanine amidase AmiA Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36548 (Residues: 35-289; Coverage: 100%)
Gene names: JW2428, amiA, b2435, yfeE
Sequence domains: N-acetylmuramoyl-L-alanine amidase

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Unit cell:
a: 59.515Å b: 73.899Å c: 115.563Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.236
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Activator-induced conformational changes regulate division-associated peptidoglycan amidases.
Cook et al. (2023)