› 8bmx

X-ray diffraction
3.72Å resolution

Bacteroides thetaiotaomicron B12 TonB dependent transporter in complex with a surface lipoprotein

Released:
DOI: 10.2210/pdb8bmx/pdb
PDB entry 8bmx coloured by chain, front view.
PDB entry 8bmx coloured by chain, side view.
PDB entry 8bmx coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
BtuB TonB-dependent transporters and BtuG surface lipoproteins form stable complexes for vitamin B12 uptake in gut Bacteroides.
Abellon-Ruiz J, Jana K, Silale A, Frey AM, Baslé A, Trost M, Kleinekathöfer U, van den Berg B
Nat Commun 14 4714 (2023)
PMID: 37543597

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240296 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TonB-linked outer membrane receptor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 698 amino acids
Theoretical weight: 79.38 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Bacteroides thetaiotaomicron VPI-5482
UniProt:
  • Canonical: Q8A6D1 (Residues: 1-692; Coverage: 100%)
Gene name: BT_1953
Sequence domains:
Surface layer protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 366 amino acids
Theoretical weight: 42.61 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
UniProt:
  • Canonical: Q8A6D0 (Residues: 1-366; Coverage: 100%)
Gene name: BT_1954
Sequence domains: Domain of unknown function (DUF5074)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 280.25Å b: 154.3Å c: 107.51Å
α: 90° β: 106.96° γ: 90°
R-values:
R R work R free
0.256 0.253 0.318
Expression system: Bacteroides thetaiotaomicron VPI-5482

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