› 8tnn

X-ray diffraction
3.36Å resolution

Crystal structure of Epstein-Barr virus gH/gL/gp42 in complex with gp42 antibody A10

Released:
DOI: 10.2210/pdb8tnn/pdb
PDB entry 8tnn coloured by chain, front view.
PDB entry 8tnn coloured by chain, side view.
PDB entry 8tnn coloured by chain, top view.
Source organisms:
Primary publication:
Epstein-Barr virus gp42 antibodies reveal sites of vulnerability for receptor binding and fusion to B cells.
Bu W, Kumar A, Board NL, Kim J, Dowdell K, Zhang S, Lei Y, Hostal A, Krogmann T, Wang Y, Pittaluga S, Marcotrigiano J, Cohen JI
Immunity 57 559-573.e6 (2024)
PMID: 38479361

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-242263 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Envelope glycoprotein H Chains: A, D
Molecule details ›
Chains: A, D
Length: 658 amino acids
Theoretical weight: 72.83 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Homo sapiens
UniProt:
  • Canonical: P03231 (Residues: 17-674; Coverage: 95%)
Gene names: BXLF2, gH
Sequence domains:
Envelope glycoprotein L Chains: B, E
Molecule details ›
Chains: B, E
Length: 112 amino acids
Theoretical weight: 12.48 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Homo sapiens
UniProt:
  • Canonical: P03212 (Residues: 24-135; Coverage: 97%)
Gene names: BKRF2, gL
Sequence domains: Viral glycoprotein L
Soluble gp42 Chains: C, F
Molecule details ›
Chains: C, F
Length: 191 amino acids
Theoretical weight: 21.63 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Homo sapiens
UniProt:
  • Canonical: P03205 (Residues: 33-223; Coverage: 86%)
Gene name: BZLF2
A10 light chain Chains: G, I
Molecule details ›
Chains: G, I
Length: 213 amino acids
Theoretical weight: 22.65 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens
A10 heavy chain Chains: H, J
Molecule details ›
Chains: H, J
Length: 234 amino acids
Theoretical weight: 25.37 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 88.75Å b: 138.96Å c: 316.264Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.281
Expression system: Homo sapiens

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