› 7y97

X-ray diffraction
2.36Å resolution

Crystal structure of CYP109B4 from Bacillus Sonorensis

Released:
DOI: 10.2210/pdb7y97/pdb
PDB entry 7y97 coloured by chain, front view.
PDB entry 7y97 coloured by chain, side view.
PDB entry 7y97 coloured by chain, top view.
Source organism: Bacillus sonorensis L12
Primary publication:
Rationally Controlling Selective Steroid Hydroxylation via Scaffold Sampling of a P450 Family
Zhang X, Shen P, Zhao J, Chen Y, Li X, Huang JW, Zhang L, Li Q, Gao C, Xing Q, Chen CC, Guo RT, Li A
ACS Catalysis 13 1280-1289 (2023)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 monooxygenase YjiB Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 46.49 KDa
Source organism: Bacillus sonorensis L12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M5PFT9 (Residues: 1-405; Coverage: 100%)
Gene name: BSONL12_06623
Sequence domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P21
Unit cell:
a: 71.987Å b: 90.543Å c: 72.029Å
α: 90° β: 102.26° γ: 90°
R-values:
R R work R free
0.22 0.218 0.26
Expression system: Escherichia coli BL21(DE3)

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