› 7y3g

Electron Microscopy
2.77Å resolution

Cryo-EM structure of a class A orphan GPCR

Released:
DOI: 10.2210/pdb7y3g/pdb
PDB entry 7y3g coloured by chain, front view.
PDB entry 7y3g coloured by chain, side view.
PDB entry 7y3g coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into the constitutive activity of human orphan receptor GPR12.
Li H, Zhang J, Yu Y, Luo F, Wu L, Liu J, Chen N, Liu Z, Hua T
Sci Bull (Beijing) 68 95-104 (2023)
PMID: 36593162
Related structures: EMD-33594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225655 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 157 amino acids
Theoretical weight: 17.06 KDa
Source organism: Lama glama
Expression system: Escherichia coli
G-protein coupled receptor 12 Chain: R
Molecule details ›
Chain: R
Length: 378 amino acids
Theoretical weight: 42.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47775 (Residues: 1-334; Coverage: 100%)
Gene name: GPR12
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.77Å
Relevant EMDB volumes: EMD-33594
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

3 citation in other articles

An inverse agonist of orphan receptor GPR61 acts by a G protein-competitive allosteric mechanism.
Lees et al. (2023)
2 more