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The nucleoplasmic phase of pre-40S formation prior to nuclear export.

Cheng J, Lau B, Thoms M, Ameismeier M, Berninghausen O, Hurt E, Beckmann R
Nucleic Acids Res (2022)
Related entries: 7wtn, 7wto, 7wtp, 7wtq, 7wtr, 7wts, 7wtt, 7wtu, 7wtw, 7wtx, 7wtz, 7wu0

Cited: 6 times
EuropePMC logo PMID: 36321656

Abstract

Biogenesis of the small ribosomal subunit in eukaryotes starts in the nucleolus with the formation of a 90S precursor and ends in the cytoplasm. Here, we elucidate the enigmatic structural transitions of assembly intermediates from human and yeast cells during the nucleoplasmic maturation phase. After dissociation of all 90S factors, the 40S body adopts a close-to-mature conformation, whereas the 3' major domain, later forming the 40S head, remains entirely immature. A first coordination is facilitated by the assembly factors TSR1 and BUD23-TRMT112, followed by re-positioning of RRP12 that is already recruited early to the 90S for further head rearrangements. Eventually, the uS2 cluster, CK1 (Hrr25 in yeast) and the export factor SLX9 associate with the pre-40S to provide export competence. These exemplary findings reveal the evolutionary conserved mechanism of how yeast and humans assemble the 40S ribosomal subunit, but reveal also a few minor differences.

Articles - 7wtv mentioned but not cited (1)

  1. The nucleoplasmic phase of pre-40S formation prior to nuclear export. Cheng J, Lau B, Thoms M, Ameismeier M, Berninghausen O, Hurt E, Beckmann R. Nucleic Acids Res 50 11924-11937 (2022)


Reviews citing this publication (2)

  1. Ribosome biogenesis factors-from names to functions. Dörner K, Ruggeri C, Zemp I, Kutay U. EMBO J 42 e112699 (2023)
  2. Ribosomal Protein uS5 and Friends: Protein-Protein Interactions Involved in Ribosome Assembly and Beyond. Landry-Voyer AM, Mir Hassani Z, Avino M, Bachand F. Biomolecules 13 853 (2023)

Articles citing this publication (3)

  1. A disease associated mutant reveals how Ltv1 orchestrates RP assembly and rRNA folding of the small ribosomal subunit head. Blomqvist EK, Huang H, Karbstein K. PLoS Genet 19 e1010862 (2023)
  2. Impact of the yeast S0/uS2-cluster ribosomal protein rpS21/eS21 on rRNA folding and the architecture of small ribosomal subunit precursors. Pöll G, Griesenbeck J, Tschochner H, Milkereit P. PLoS One 18 e0283698 (2023)
  3. Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2. Ikeuchi K, Ivic N, Buschauer R, Cheng J, Fröhlich T, Matsuo Y, Berninghausen O, Inada T, Becker T, Beckmann R. Nat Commun 14 2730 (2023)


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