› 7s7v

X-ray diffraction
2.5Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
iNicSnFR 3.0 Fluorescent Nicotine Sensor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 537 amino acids
Theoretical weight: 60.33 KDa
Source organism: Thermoanaerobacter sp. X513
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 4 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 81.189Å b: 86.865Å c: 91.83Å
α: 75.72° β: 85.56° γ: 85.28°
R-values:
R R work R free
0.233 0.232 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 mentions without citation

Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands.
Nichols et al. (2022)
2 more