› 7qoe

X-ray diffraction
1.2Å resolution

Structure of a small alarmone hydrolase from Leptospira levettii

Released:
DOI: 10.2210/pdb7qoe/pdb
PDB entry 7qoe coloured by chain, front view.
PDB entry 7qoe coloured by chain, side view.
PDB entry 7qoe coloured by chain, top view.
Source organism: Leptospira levettii
Primary publication:
Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response.
Bisiak F, Chrenková A, Zhang SD, Pedersen JN, Otzen DE, Zhang YE, Brodersen DE
J Biol Chem 102142 (2022)
PMID: 35714769

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HD/PDEase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.36 KDa
Source organism: Leptospira levettii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2N0AXP5 (Residues: 1-196; Coverage: 100%)
Gene name: CH368_08595
Sequence domains: HD domain

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P42212
Unit cell:
a: 98.708Å b: 98.708Å c: 40.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Metabolic Promiscuity of an Orphan Small Alarmone Hydrolase Facilitates Bacterial Environmental Adaptation.
Fung et al. (2022)
1 more

2 mentions without citation

Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response.
Bisiak et al. (2022)
1 more