› 7ort

X-ray diffraction
2.33Å resolution

Ternary complex of 14-3-3 sigma, p27pT198 phosphopeptide, and WQ176

Released:
DOI: 10.2210/pdb7ort/pdb
PDB entry 7ort coloured by chain, front view.
PDB entry 7ort coloured by chain, side view.
PDB entry 7ort coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Centorrino F, Wu Q, Ottmann C

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152126 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Cyclin-dependent kinase inhibitor 1B Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P46527 (Residues: 187-198; Coverage: 6%)
Gene names: CDKN1B, KIP1, p27

Ligands and Environments

3 bound ligands:
Ligand 0HP 1 x 0HP
Ligand MG 2 x MG
Ligand CL 1 x CL
2 modified residues:
Ligand CSO 1 x CSO
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: C2221
Unit cell:
a: 82.579Å b: 112.025Å c: 62.632Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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