› 7obz

X-ray diffraction
2Å resolution

Structure of RsLOV D109G

Released:
DOI: 10.2210/pdb7obz/pdb
PDB entry 7obz coloured by chain, front view.
PDB entry 7obz coloured by chain, side view.
PDB entry 7obz coloured by chain, top view.
Source organism: Cereibacter sphaeroides ATCC 17025
Primary publication:
A Light-Oxygen-Voltage Receptor Integrates Light and Temperature.
Dietler J, Schubert R, Krafft TGA, Meiler S, Kainrath S, Richter F, Schweimer K, Weyand M, Janovjak H, Möglich A
J Mol Biol 433 167107 (2021)
PMID: 34146595

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAS domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 176 amino acids
Theoretical weight: 19.4 KDa
Source organism: Cereibacter sphaeroides ATCC 17025
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M1E1F8 (Residues: 1-176; Coverage: 100%)
Sequence domains: PAS domain

Ligands and Environments

4 bound ligands:
Ligand FMN 6 x FMN
Ligand CL 13 x CL
Ligand SPD 5 x SPD
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 90.96Å b: 65.8Å c: 122.72Å
α: 90° β: 94° γ: 90°
R-values:
R R work R free
0.219 0.218 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Toward Multiplexed Optogenetic Circuits.
Dwijayanti et al. (2021)
3 more