› 7nfg

X-ray diffraction
1.09Å resolution

A hexameric barrel state of a de novo coiled-coil assembly: CC-Type2-(LaId)4-L14A.

Released:
DOI: 10.2210/pdb7nfg/pdb
PDB entry 7nfg coloured by chain, front view.
PDB entry 7nfg coloured by chain, side view.
PDB entry 7nfg coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Differential sensing with arrays of de novo designed peptide assemblies.
Dawson WM, Shelley KL, Fletcher JM, Scott DA, Lombardi L, Rhys GG, LaGambina TJ, Obst U, Burton AJ, Cross JA, Davies G, Martin FJO, Wiseman FJ, Brady RL, Tew D, Wood CW, Woolfson DN
Nat Commun 14 383 (2023)
PMID: 36693847

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CC-Type2-(LaId)4-L14A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 32 amino acids
Theoretical weight: 3.19 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 31.67Å b: 57.24Å c: 91.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.17
Expression system: Not provided

Quick links

Citations

2 review citations

Understanding a protein fold: The physics, chemistry, and biology of α-helical coiled coils.
Woolfson DN. (2023)
1 more