› 7kqr

X-ray diffraction
1.89Å resolution

A 1.89-A resolution substrate-bound crystal structure of heme-dependent tyrosine hydroxylase from S. sclerotialus

Released:
DOI: 10.2210/pdb7kqr/pdb
PDB entry 7kqr coloured by chain, front view.
PDB entry 7kqr coloured by chain, side view.
PDB entry 7kqr coloured by chain, top view.
Source organism: Streptomyces sclerotialus
Primary publication:
Molecular Rationale for Partitioning between C-H and C-F Bond Activation in Heme-Dependent Tyrosine Hydroxylase.
Wang Y, Davis I, Shin I, Xu H, Liu A
J Am Chem Soc 143 4680-4693 (2021)
PMID: 33734681

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme-dependent L-tyrosine hydroxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 34.62 KDa
Source organism: Streptomyces sclerotialus
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand HEM 2 x HEM
Ligand TYR 2 x TYR
Ligand BTB 1 x BTB
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 47.707Å b: 129.781Å c: 48.44Å
α: 90° β: 93.87° γ: 90°
R-values:
R R work R free
0.168 0.166 0.207
Expression system: Escherichia coli

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Citations

2 citation in other articles

Contrasting Mechanisms of Aromatic and Aryl-Methyl Substituent Hydroxylation by the Rieske Monooxygenase Salicylate 5-Hydroxylase.
Rogers et al. (2023)
1 more

2 mentions without citation

A new regime of heme-dependent aromatic oxygenase superfamily.
Shin et al. (2021)
1 more