› 7f9p

X-ray diffraction
1.57Å resolution

Toxoplasma gondii Prolyl-tRNA Synthetase (TgPRS) in Complex with inhibitor L95 and azetidine

Released:
DOI: 10.2210/pdb7f9p/pdb
PDB entry 7f9p coloured by chain, front view.
PDB entry 7f9p coloured by chain, side view.
PDB entry 7f9p coloured by chain, top view.
Source organism: Toxoplasma gondii
Entry authors: Manickam Y, Malhotra N, Sharma A

Function and Biology Details

Reaction catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
proline--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 57.94 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A7J6JUK2 (Residues: 215-711; Coverage: 70%)
Gene name: TGRH88_057780
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand JE6 1 x JE6
Ligand 02A 1 x 02A
Ligand MES 1 x MES
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 151.13Å b: 75.495Å c: 110.166Å
α: 90° β: 152.24° γ: 90°
R-values:
R R work R free
0.177 0.176 0.202
Expression system: Escherichia coli

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