› 7arw

X-ray diffraction
1.31Å resolution

Structure of human ARH3 E41A bound to alpha-NAD+ and magnesium

Released:
DOI: 10.2210/pdb7arw/pdb
PDB entry 7arw coloured by chain, front view.
PDB entry 7arw coloured by chain, side view.
PDB entry 7arw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanistic insights into the three steps of poly(ADP-ribosylation) reversal.
Rack JGM, Liu Q, Zorzini V, Voorneveld J, Ariza A, Honarmand Ebrahimi K, Reber JM, Krassnig SC, Ahel D, van der Marel GA, Mangerich A, McCullagh JSO, Filippov DV, Ahel I
Nat Commun 12 4581 (2021)
PMID: 34321462

Function and Biology Details

Reaction catalysed: 
Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylhydrolase ARH3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 37.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NX46 (Residues: 19-363; Coverage: 95%)
Gene names: ADPRHL2, ADPRS, ARH3
Sequence domains: ADP-ribosylglycohydrolase

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand 8NA 2 x 8NA
Ligand EDO 12 x EDO
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P1
Unit cell:
a: 44.746Å b: 66.112Å c: 70.055Å
α: 115.8° β: 94.98° γ: 104.07°
R-values:
R R work R free
0.167 0.166 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

ADP-Ribosylation as Post-Translational Modification of Proteins: Use of Inhibitors in Cancer Control.
Poltronieri et al. (2021)
10 more