› 7akp

X-ray diffraction
2.59Å resolution

Crystal structure of E. coli RNA helicase HrpA-D305A

Released:
DOI: 10.2210/pdb7akp/pdb
PDB entry 7akp coloured by chain, front view.
PDB entry 7akp coloured by chain, side view.
PDB entry 7akp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Large-scale ratcheting in a bacterial DEAH/RHA-type RNA helicase that modulates antibiotics susceptibility.
Grass LM, Wollenhaupt J, Barthel T, Parfentev I, Urlaub H, Loll B, Klauck E, Antelmann H, Wahl MC
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 34290142

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase HrpA Chain: A
Molecule details ›
Chain: A
Length: 787 amino acids
Theoretical weight: 89.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P43329 (Residues: 1-783; Coverage: 60%)
Gene names: JW5905, b1413, hrpA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 39.894Å b: 114.902Å c: 94.614Å
α: 90° β: 101.937° γ: 90°
R-values:
R R work R free
0.22 0.211 0.239
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Regulation of the DEAH/RHA helicase Prp43 by the G-patch factor Pfa1.
Enders et al. (2022)
3 more