7rua

Electron Microscopy
3.4Å resolution

Metazoan pre-targeting GET complex (cBUGG-out)

Released:
Source organisms:
Primary publication:
Structural insights into metazoan pretargeting GET complexes.
Nat Struct Mol Biol 28 1029-1037 (2021)
PMID: 34887561
Related structures: EMD-24701

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-145832 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATPase GET3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 38.57 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6IQE5 (Residues: 1-341; Coverage: 100%)
Gene names: asna1, get3, si:dkey-121b10.2, zgc:86799
Sequence domains: Anion-transporting ATPase
Golgi to ER traffic protein 4 homolog Chains: C, F
Molecule details ›
Chains: C, F
Length: 333 amino acids
Theoretical weight: 37.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7L5D6 (Residues: 1-327; Coverage: 100%)
Gene names: C7orf20, CEE, CGI-20, GET4, TRC35
Sequence domains: Golgi to ER traffic protein 4
Large proline-rich protein BAG6 Chains: D, G
Molecule details ›
Chains: D, G
Length: 132 amino acids
Theoretical weight: 14.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46379 (Residues: 1004-1132; Coverage: 11%)
Gene names: BAG6, BAT3, G3
Sequence domains: Bag6, BAG-similar domain
Ubiquitin-like protein 4A Chains: E, H
Molecule details ›
Chains: E, H
Length: 165 amino acids
Theoretical weight: 18.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11441 (Residues: 1-157; Coverage: 100%)
Gene names: DXS254E, GDX, UBL4, UBL4A
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-24701
Expression system: Escherichia coli