› 7kmr

X-ray diffraction
1.51Å resolution

Crystal structure analysis of human KRAS mutant

Released:
DOI: 10.2210/pdb7kmr/pdb
PDB entry 7kmr coloured by chain, front view.
PDB entry 7kmr coloured by chain, side view.
PDB entry 7kmr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of GTPase function by autophosphorylation.
Johnson CW, Seo HS, Terrell EM, Yang MH, KleinJan F, Gebregiworgis T, Gasmi-Seabrook GMC, Geffken EA, Lakhani J, Song K, Bashyal P, Popow O, Paulo JA, Liu A, Mattos C, Marshall CB, Ikura M, Morrison DK, Dhe-Paganon S, Haigis KM
Mol Cell 82 950-968.e14 (2022)
PMID: 35202574

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas Chain: A
Molecule details ›
Chain: A
Length: 210 amino acids
Theoretical weight: 23.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-186; Coverage: 98%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P321
Unit cell:
a: 78.4Å b: 78.4Å c: 55.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.197
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Predictive and Prognostic Role of RAS-RAF-MEK-ERK Pathway Alterations in Breast Cancer: Revision of the Literature and Comparison with the Analysis of Cancer Genomic Datasets.
Rocca et al. (2022)
1 more

1 mention without citation

Regulation of GTPase function by autophosphorylation.
Johnson et al. (2022)